In vivo natural killer cell depletion during primary simian immunodeficiency virus infection in rhesus monkeys - PubMed (original) (raw)

Comparative Study

. 2008 Jul;82(13):6758-61.

doi: 10.1128/JVI.02277-07. Epub 2008 Apr 23.

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Comparative Study

In vivo natural killer cell depletion during primary simian immunodeficiency virus infection in rhesus monkeys

Elisa I Choi et al. J Virol. 2008 Jul.

Abstract

The contribution of natural killer (NK) cells to the immune containment of human immunodeficiency virus infection remains undefined. To directly assess the role of NK cells in an AIDS animal model, we depleted rhesus monkeys of >88% of CD3(-) CD16(+) CD159a(+) NK cells at the time of primary simian immunodeficiency virus (SIV) infection by using anti-CD16 antibody. During the first 11 days following SIV inoculation, when NK cell depletion was most profound, a trend toward higher levels of SIV replication was noted in NK cell-depleted monkeys compared to those in control monkeys. However, this treatment did not result in significant changes in the overall levels or kinetics of plasma viral RNA or affect the SIV-induced central memory CD4(+) T-lymphocyte loss. These findings are consistent with a limited role for cytotoxic CD16(+) NK cells in the control of primary SIV viremia.

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Figures

FIG. 1.

FIG. 1.

Monoclonal anti-CD16 antibody (Ab) administration depleted NK cells from blood during primary SIV infection. Rhesus monkeys were administered either anti-CD16 (red) or a control monoclonal antibody (blue) and infected 1 day later with SIV. NK cell numbers (A) and percentages (B) were monitored by flow cytometric analysis of blood lymphocytes, with NK cells defined as CD3− CD16+ CD159A+. Data are shown as medians ± IQRs. An asterisk indicates a significant difference between groups (Kruskal-Wallis test/Dunn's multiple-comparison posttest, P < 0.05).

FIG. 2.

FIG. 2.

NK cell depletion during primary SIV infection had minimal effects on plasma viral RNA levels and virally induced loss of CD4 central memory cells. (A) Plasma viral RNA levels are shown for the anti-CD16 (red) and control (blue) monoclonal antibody-treated monkeys. (B) The peripheral blood central memory (CM) CD4+ T lymphocytes were defined as CD3+ CD4+ CD28+ CD95+. Significant differences were not detected between groups at any time point (Kruskal-Wallis test/Dunn's multiple-comparison posttest, P < 0.05).

FIG. 3.

FIG. 3.

Area-under-the-curve analysis of plasma viral load during primary SIV viremia. Plasma viral load was analyzed by integrating the area under the curve for the first 11 days of infection leading up to the peak of replication (A) and for the 15 days following the peak of viremia (days 11 to 27) as virus replication declined to the set point (B). Significant differences were not detected between groups (Mann-Whitney U test, P < 0.05).

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