Vaccinia virus uses macropinocytosis and apoptotic mimicry to enter host cells - PubMed (original) (raw)

. 2008 Apr 25;320(5875):531-5.

doi: 10.1126/science.1155164.

Affiliations

• PMID: 18436786
• DOI: 10.1126/science.1155164

Vaccinia virus uses macropinocytosis and apoptotic mimicry to enter host cells

Jason Mercer et al. Science. 2008.

Abstract

Viruses employ many different strategies to enter host cells. Vaccinia virus, a prototype poxvirus, enters cells in a pH-dependent fashion. Live cell imaging showed that fluorescent virus particles associated with and moved along filopodia to the cell body, where they were internalized after inducing the extrusion of large transient membrane blebs. p21-activated kinase 1 (PAK1) was activated by the virus, and the endocytic process had the general characteristics of macropinocytosis. The induction of blebs, the endocytic event, and infection were all critically dependent on the presence of exposed phosphatidylserine in the viral membrane, which suggests that vaccinia virus uses apoptotic mimicry to enter cells.

PubMed Disclaimer

Comment in

• Cell biology. A one-sided signal.
  Fairn GD, Grinstein S. Fairn GD, et al. Science. 2008 Apr 25;320(5875):458-60. doi: 10.1126/science.1158173. Science. 2008. PMID: 18436763 No abstract available.

Similar articles

• Apoptotic mimicry: phosphatidylserine-mediated macropinocytosis of vaccinia virus.
  Mercer J, Helenius A. Mercer J, et al. Ann N Y Acad Sci. 2010 Oct;1209:49-55. doi: 10.1111/j.1749-6632.2010.05772.x. Ann N Y Acad Sci. 2010. PMID: 20958316
• Cell biology. A one-sided signal.
  Fairn GD, Grinstein S. Fairn GD, et al. Science. 2008 Apr 25;320(5875):458-60. doi: 10.1126/science.1158173. Science. 2008. PMID: 18436763 No abstract available.
• Vaccinia virus strains use distinct forms of macropinocytosis for host-cell entry.
  Mercer J, Knébel S, Schmidt FI, Crouse J, Burkard C, Helenius A. Mercer J, et al. Proc Natl Acad Sci U S A. 2010 May 18;107(20):9346-51. doi: 10.1073/pnas.1004618107. Epub 2010 May 3. Proc Natl Acad Sci U S A. 2010. PMID: 20439710 Free PMC article.
• Viral apoptotic mimicry.
  Amara A, Mercer J. Amara A, et al. Nat Rev Microbiol. 2015 Aug;13(8):461-9. doi: 10.1038/nrmicro3469. Epub 2015 Jun 8. Nat Rev Microbiol. 2015. PMID: 26052667 Free PMC article. Review.
• Come in and take your coat off - how host cells provide endocytosis for virus entry.
  Schelhaas M. Schelhaas M. Cell Microbiol. 2010 Oct;12(10):1378-88. doi: 10.1111/j.1462-5822.2010.01510.x. Cell Microbiol. 2010. PMID: 20678171 Review.

Cited by

• Nanoengineering-armed oncolytic viruses drive antitumor response: progress and challenges.
  Zhang Y, Shi X, Shen Y, Dong X, He R, Chen G, Zhang Y, Tan H, Zhang K. Zhang Y, et al. MedComm (2020). 2024 Oct 10;5(10):e755. doi: 10.1002/mco2.755. eCollection 2024 Oct. MedComm (2020). 2024. PMID: 39399642 Free PMC article. Review.
• The ability of human TIM1 to bind phosphatidylethanolamine enhances viral uptake and efferocytosis compared to rhesus and mouse orthologs.
  Zhang L, Kitzmiller CE, Richard AS, Popli S, Choe H. Zhang L, et al. bioRxiv [Preprint]. 2024 Jul 29:2024.07.29.605603. doi: 10.1101/2024.07.29.605603. bioRxiv. 2024. PMID: 39131348 Free PMC article. Preprint.
• Multi-omics characterization of the monkeypox virus infection.
  Huang Y, Bergant V, Grass V, Emslander Q, Hamad MS, Hubel P, Mergner J, Piras A, Krey K, Henrici A, Öllinger R, Tesfamariam YM, Dalla Rosa I, Bunse T, Sutter G, Ebert G, Schmidt FI, Way M, Rad R, Bowie AG, Protzer U, Pichlmair A. Huang Y, et al. Nat Commun. 2024 Aug 8;15(1):6778. doi: 10.1038/s41467-024-51074-6. Nat Commun. 2024. PMID: 39117661 Free PMC article.
• DF-1-Derived exosomes mediate transmission of reticuloendotheliosis virus and resist REV-specific antibodies.
  Wang Z, Cui H, Zhang Y, Sun W, Yang W, Zhao P. Wang Z, et al. Virol J. 2024 Aug 6;21(1):177. doi: 10.1186/s12985-024-02445-4. Virol J. 2024. PMID: 39107806 Free PMC article.
• Macropinocytosis Is the Principal Uptake Mechanism of Antigen-Presenting Cells for Allergen-Specific Virus-like Nanoparticles.
  Kraus A, Kratzer B, Sehgal ANA, Trapin D, Khan M, Boucheron N, Pickl WF. Kraus A, et al. Vaccines (Basel). 2024 Jul 18;12(7):797. doi: 10.3390/vaccines12070797. Vaccines (Basel). 2024. PMID: 39066435 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Other Literature Sources

  • H1 Connect
  • The Lens - Patent Citations

• Research Materials

  • NCI CPTC Antibody Characterization Program