Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system - PubMed (original) (raw)

doi: 10.1038/nsmb.1426. Epub 2008 Apr 27.

Affiliations

PMID: 18438417
DOI: 10.1038/nsmb.1426

Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system

Konstantin V Korotkov et al. Nat Struct Mol Biol. 2008 May.

Abstract

Gram-negative bacteria translocate various proteins including virulence factors across their outer membrane via type 2 secretion systems (T2SSs). T2SSs are thought to contain a pseudopilus, a subcomplex formed by one major and several minor pseudopilins. We report the crystal structure of the complex formed by three minor pseudopilins from enterotoxigenic Escherichia coli. The GspK-GspI-GspJ complex has quasihelical characteristics and an architecture consistent with a localization at the pseudopilus tip. The alpha-domain of GspK has a previously unobserved fold with an unexpected dinuclear metal binding site. The area surrounding its disulfide bridge is conserved and might interact with other T2SS components or with secreted proteins.

PubMed Disclaimer

Comment in

The type II secretion arrowhead: the structure of GspI-GspJ-GspK.
Forest KT. Forest KT. Nat Struct Mol Biol. 2008 May;15(5):428-30. doi: 10.1038/nsmb0508-428. Nat Struct Mol Biol. 2008. PMID: 18461043 No abstract available.

Cited by

Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system.
Horváthová L, Žárský V, Pánek T, Derelle R, Pyrih J, Motyčková A, Klápšťová V, Vinopalová M, Marková L, Voleman L, Klimeš V, Petrů M, Vaitová Z, Čepička I, Hryzáková K, Harant K, Gray MW, Chami M, Guilvout I, Francetic O, Franz Lang B, Vlček Č, Tsaousis AD, Eliáš M, Doležal P. Horváthová L, et al. Nat Commun. 2021 May 19;12(1):2947. doi: 10.1038/s41467-021-23046-7. Nat Commun. 2021. PMID: 34011950 Free PMC article.
Structures of type IV pilins from Thermus thermophilus demonstrate similarities with type II secretion system pseudopilins.
Karuppiah V, Thistlethwaite A, Derrick JP. Karuppiah V, et al. J Struct Biol. 2016 Dec;196(3):375-384. doi: 10.1016/j.jsb.2016.08.006. Epub 2016 Sep 6. J Struct Biol. 2016. PMID: 27612581 Free PMC article.
Many substrates and functions of type II secretion: lessons learned from Legionella pneumophila.
Cianciotto NP. Cianciotto NP. Future Microbiol. 2009 Sep;4(7):797-805. doi: 10.2217/fmb.09.53. Future Microbiol. 2009. PMID: 19722835 Free PMC article. Review.
Structure of Clostridium difficile PilJ exhibits unprecedented divergence from known type IV pilins.
Piepenbrink KH, Maldarelli GA, de la Peña CF, Mulvey GL, Snyder GA, De Masi L, von Rosenvinge EC, Günther S, Armstrong GD, Donnenberg MS, Sundberg EJ. Piepenbrink KH, et al. J Biol Chem. 2014 Feb 14;289(7):4334-45. doi: 10.1074/jbc.M113.534404. Epub 2013 Dec 21. J Biol Chem. 2014. PMID: 24362261 Free PMC article.
Structure of a heteropolymeric type 4 pilus from a monoderm bacterium.
Anger R, Pieulle L, Shahin M, Valette O, Le Guenno H, Kosta A, Pelicic V, Fronzes R. Anger R, et al. Nat Commun. 2023 Nov 6;14(1):7143. doi: 10.1038/s41467-023-42872-5. Nat Commun. 2023. PMID: 37932265 Free PMC article.

Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion system - PubMed (original) (raw)