Polyomavirus replication in mice: influences of VP1 type and route of inoculation - PubMed (original) (raw)

Comparative Study

Polyomavirus replication in mice: influences of VP1 type and route of inoculation

T W Dubensky et al. J Virol. 1991 Jan.

Abstract

Patterns of polyomavirus replication and spread have been studied following inoculation of virus into newborn mice. Levels of virus replication in different tissues were followed in situ by using whole mouse section blots and immunoperoxidase staining for the major capsid protein VP1, as well as by tissue extraction and direct quantitation of viral DNA and infectious virus. Patterns of replication and spread were compared between the "high tumor" strain (inducing a high incidence of tumors) PTA and and the "low tumor" strain (inducing a low incidence of tumors) RA, following different routes of inoculation. The ability to induce a high tumor profile correlated with the ability to establish disseminated productive infection, with the kidney as a major site of amplification. Furthermore, results with PTA-RA recombinant viruses and site-directed mutants showed that the VP1 specificity of PTA, demonstrated earlier to be a critical determinant for induction of a high tumor profile (R. Freund, A. Calderone, C. J. Dawe, and T. L. Benjamin, J. Virol. 65:335-341, 1991), is also critical for amplification in the kidney and for establishment of disseminated infections.

PubMed Disclaimer

Cited by

Adaptive mutations in the JC virus protein capsid are associated with progressive multifocal leukoencephalopathy (PML).
Sunyaev SR, Lugovskoy A, Simon K, Gorelik L. Sunyaev SR, et al. PLoS Genet. 2009 Feb;5(2):e1000368. doi: 10.1371/journal.pgen.1000368. Epub 2009 Feb 6. PLoS Genet. 2009. PMID: 19197354 Free PMC article.
Cell-type specific activation of the polyomavirus F9-1 regulatory region in transgenic mice.
Neubauer A, Manitz MP, Napirei M, Krippl B. Neubauer A, et al. Transgenic Res. 1996 Nov;5(6):373-83. doi: 10.1007/BF01980202. Transgenic Res. 1996. PMID: 8840520
Ganglioside and Non-ganglioside Mediated Host Responses to the Mouse Polyomavirus.
You J, O'Hara SD, Velupillai P, Castle S, Levery S, Garcea RL, Benjamin T. You J, et al. PLoS Pathog. 2015 Oct 16;11(10):e1005175. doi: 10.1371/journal.ppat.1005175. eCollection 2015 Oct. PLoS Pathog. 2015. PMID: 26474471 Free PMC article.
Predominant binding of Theiler's viruses to a 34-kilodalton receptor protein on susceptible cell lines.
Kilpatrick DR, Lipton HL. Kilpatrick DR, et al. J Virol. 1991 Oct;65(10):5244-9. doi: 10.1128/JVI.65.10.5244-5249.1991. J Virol. 1991. PMID: 1895381 Free PMC article.
A tumor host range selection procedure identifies p150(sal2) as a target of polyoma virus large T antigen.
Li D, Dower K, Ma Y, Tian Y, Benjamin TL. Li D, et al. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14619-24. doi: 10.1073/pnas.251447198. Proc Natl Acad Sci U S A. 2001. PMID: 11734654 Free PMC article.

References

1. J Virol. 1984 May;50(2):541-6 - PubMed
1. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 - PubMed
1. Virology. 1975 Aug;66(2):372-84 - PubMed
1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
1. J Exp Med. 1959 Apr 1;109(4):379-91 - PubMed

Polyomavirus replication in mice: influences of VP1 type and route of inoculation - PubMed (original) (raw)