Varp interacts with Rab38 and functions as its potential effector - PubMed (original) (raw)
. 2008 Jul 18;372(1):162-7.
doi: 10.1016/j.bbrc.2008.05.017. Epub 2008 May 12.
Affiliations
- PMID: 18477474
- DOI: 10.1016/j.bbrc.2008.05.017
Varp interacts with Rab38 and functions as its potential effector
Fang Wang et al. Biochem Biophys Res Commun. 2008.
Abstract
Varp, a novel protein containing a VPS9 domain and ankyrin repeats, can function as a guanine nucleotide exchange factor (GEF) of Rab21. We previously reported that Varp plays an important role in the regulation of endosome dynamics. To further investigate the function of Varp, a yeast two-hybrid screen was performed and Rab38 was identified as a Varp-associated protein. We demonstrate that Varp physically interacts with Rab38, and preferentially binds to the active GTP-bound form of Rab38 both in vitro and in vivo. Furthermore, Varp was shown to be recruited to Rab38-positive organelles in an ankyrin-repeat 1 (ANK1)-dependent manner. Our data demonstrate that Varp is a potential effector of Rab38. Together with our previous study, we propose Varp serves as both an effector and a GEF by interacting with different Rabs in mammalian cells.
Similar articles
- The Rab21-GEF activity of Varp, but not its Rab32/38 effector function, is required for dendrite formation in melanocytes.
Ohbayashi N, Yatsu A, Tamura K, Fukuda M. Ohbayashi N, et al. Mol Biol Cell. 2012 Feb;23(4):669-78. doi: 10.1091/mbc.E11-04-0324. Epub 2011 Dec 14. Mol Biol Cell. 2012. PMID: 22171327 Free PMC article. - Multiple Roles of VARP in Endosomal Trafficking: Rabs, Retromer Components and R-SNARE VAMP7 Meet on VARP.
Fukuda M. Fukuda M. Traffic. 2016 Jul;17(7):709-19. doi: 10.1111/tra.12406. Epub 2016 May 13. Traffic. 2016. PMID: 27103185 Review. - Varp is a Rab21 guanine nucleotide exchange factor and regulates endosome dynamics.
Zhang X, He X, Fu XY, Chang Z. Zhang X, et al. J Cell Sci. 2006 Mar 15;119(Pt 6):1053-62. doi: 10.1242/jcs.02810. J Cell Sci. 2006. PMID: 16525121 - Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes.
Tamura K, Ohbayashi N, Maruta Y, Kanno E, Itoh T, Fukuda M. Tamura K, et al. Mol Biol Cell. 2009 Jun;20(12):2900-8. doi: 10.1091/mbc.e08-12-1161. Epub 2009 Apr 29. Mol Biol Cell. 2009. PMID: 19403694 Free PMC article. - Rab family of small GTPases: an updated view on their regulation and functions.
Homma Y, Hiragi S, Fukuda M. Homma Y, et al. FEBS J. 2021 Jan;288(1):36-55. doi: 10.1111/febs.15453. Epub 2020 Jul 1. FEBS J. 2021. PMID: 32542850 Free PMC article. Review.
Cited by
- Rab9A is required for delivery of cargo from recycling endosomes to melanosomes.
Mahanty S, Ravichandran K, Chitirala P, Prabha J, Jani RA, Setty SR. Mahanty S, et al. Pigment Cell Melanoma Res. 2016 Jan;29(1):43-59. doi: 10.1111/pcmr.12434. Pigment Cell Melanoma Res. 2016. PMID: 26527546 Free PMC article. - Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth.
Burgo A, Sotirakis E, Simmler MC, Verraes A, Chamot C, Simpson JC, Lanzetti L, Proux-Gillardeaux V, Galli T. Burgo A, et al. EMBO Rep. 2009 Oct;10(10):1117-24. doi: 10.1038/embor.2009.186. Epub 2009 Sep 11. EMBO Rep. 2009. PMID: 19745841 Free PMC article. - VARP is recruited on to endosomes by direct interaction with retromer, where together they function in export to the cell surface.
Hesketh GG, Pérez-Dorado I, Jackson LP, Wartosch L, Schäfer IB, Gray SR, McCoy AJ, Zeldin OB, Garman EF, Harbour ME, Evans PR, Seaman MNJ, Luzio JP, Owen DJ. Hesketh GG, et al. Dev Cell. 2014 Jun 9;29(5):591-606. doi: 10.1016/j.devcel.2014.04.010. Epub 2014 May 22. Dev Cell. 2014. PMID: 24856514 Free PMC article. - Melanosome Biogenesis in the Pigmentation of Mammalian Skin.
Le L, Sirés-Campos J, Raposo G, Delevoye C, Marks MS. Le L, et al. Integr Comp Biol. 2021 Oct 14;61(4):1517-1545. doi: 10.1093/icb/icab078. Integr Comp Biol. 2021. PMID: 34021746 Free PMC article. Review. - Emerging Role of Retromer in Modulating Pathogen Growth.
Elwell C, Engel J. Elwell C, et al. Trends Microbiol. 2018 Sep;26(9):769-780. doi: 10.1016/j.tim.2018.04.001. Epub 2018 Apr 24. Trends Microbiol. 2018. PMID: 29703496 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous