Comparison of electrochemical immunosensors based on gold nano materials and immunoblot techniques for detection of histidine-tagged proteins in culture medium - PubMed (original) (raw)
Comparative Study
. 2008 Oct 15;24(2):284-9.
doi: 10.1016/j.bios.2008.04.002. Epub 2008 Apr 11.
Affiliations
- PMID: 18486465
- DOI: 10.1016/j.bios.2008.04.002
Comparative Study
Comparison of electrochemical immunosensors based on gold nano materials and immunoblot techniques for detection of histidine-tagged proteins in culture medium
Michał Wasowicz et al. Biosens Bioelectron. 2008.
Abstract
In this work, the direct electrochemical determination of poly-histidine tagged proteins using immunosensor based on anti-His (C-term) antibody immobilized on gold electrodes modified with 1,6-hexanedithiol, gold colloid particles or gold nanorods is described. The recombinant histidine-tagged silk proteinase inhibitor protein (rSPI2-His(6)) expressed in Pichia system selected as antigen for this immonosensor. An electrochemical impedance spectroscopy was used as label free detection technique for immune conjugation. The gold nanorods modified electrode layer showed better analytical response than gold nano particles. The linear calibration range was observed between 10 pg/ml and 1 ng/ml with limit of detection 5 pg/ml (S/N=3). Up to four successive assay cycles with retentive sensitivity were achieved for the immunosensors regenerated with 0.2M glycine-HCl buffer, pH 2.8. The performance of this immnosensor were compared with immuoblotting techniques.
Similar articles
- New amperometric and potentiometric immunosensors based on gold nanoparticles/tris(2,2'-bipyridyl)cobalt(III) multilayer films for hepatitis B surface antigen determinations.
Tang D, Yuan R, Chai Y, Fu Y, Dai J, Liu Y, Zhong X. Tang D, et al. Biosens Bioelectron. 2005 Oct 15;21(4):539-48. doi: 10.1016/j.bios.2004.11.024. Biosens Bioelectron. 2005. PMID: 16202866 - A novel electrochemical immunosensor based on ordered Au nano-prickle clusters.
Wang S, Wu Z, Qu F, Zhang S, Shen G, Yu R. Wang S, et al. Biosens Bioelectron. 2008 Dec 1;24(4):1026-32. doi: 10.1016/j.bios.2008.08.013. Epub 2008 Aug 19. Biosens Bioelectron. 2008. PMID: 18819790 - Gold screen-printed-based impedimetric immunobiosensors for direct and sensitive Escherichia coli quantisation.
Escamilla-Gómez V, Campuzano S, Pedrero M, Pingarrón JM. Escamilla-Gómez V, et al. Biosens Bioelectron. 2009 Jul 15;24(11):3365-71. doi: 10.1016/j.bios.2009.04.047. Epub 2009 May 7. Biosens Bioelectron. 2009. PMID: 19481924 - State of the art and recent advances in immunoanalytical systems.
Marquette CA, Blum LJ. Marquette CA, et al. Biosens Bioelectron. 2006 Feb 15;21(8):1424-33. doi: 10.1016/j.bios.2004.09.037. Epub 2005 Dec 6. Biosens Bioelectron. 2006. PMID: 16337371 Review. - New analytical applications of gold nanoparticles as label in antibody based sensors.
Omidfar K, Khorsand F, Darziani Azizi M. Omidfar K, et al. Biosens Bioelectron. 2013 May 15;43:336-47. doi: 10.1016/j.bios.2012.12.045. Epub 2013 Jan 3. Biosens Bioelectron. 2013. PMID: 23356999 Review.
Cited by
- An immunosensor based on antibody binding fragments attached to gold nanoparticles for the detection of peptides derived from avian influenza hemagglutinin H5.
Jarocka U, Sawicka R, Góra-Sochacka A, Sirko A, Zagórski-Ostoja W, Radecki J, Radecka H. Jarocka U, et al. Sensors (Basel). 2014 Aug 25;14(9):15714-28. doi: 10.3390/s140915714. Sensors (Basel). 2014. PMID: 25157550 Free PMC article. - His-tag protein monitoring by a fast mix-and-measure immunoassay.
Kreisig T, Prasse AA, Zscharnack K, Volke D, Zuchner T. Kreisig T, et al. Sci Rep. 2014 Jul 8;4:5613. doi: 10.1038/srep05613. Sci Rep. 2014. PMID: 25000910 Free PMC article. - Immunosensor incorporating anti-His (C-term) IgG F(ab') fragments attached to gold nanorods for detection of His-tagged proteins in culture medium.
Wąsowicz M, Milner M, Radecka D, Grzelak K, Radecka H. Wąsowicz M, et al. Sensors (Basel). 2010;10(6):5409-24. doi: 10.3390/s100605409. Epub 2010 Jun 1. Sensors (Basel). 2010. PMID: 22219669 Free PMC article. - Impedimetric and Plasmonic Sensing of Collagen I Using a Half-Antibody-Supported, Au-Modified, Self-Assembled Monolayer System.
Gwiazda M, Bhardwaj SK, Kijeńska-Gawrońska E, Swieszkowski W, Sivasankaran U, Kaushik A. Gwiazda M, et al. Biosensors (Basel). 2021 Jul 8;11(7):227. doi: 10.3390/bios11070227. Biosensors (Basel). 2021. PMID: 34356698 Free PMC article. - Impact of nanotechnology on conventional and artificial intelligence-based biosensing strategies for the detection of viruses.
Ramalingam M, Jaisankar A, Cheng L, Krishnan S, Lan L, Hassan A, Sasmazel HT, Kaji H, Deigner HP, Pedraz JL, Kim HW, Shi Z, Marrazza G. Ramalingam M, et al. Discov Nano. 2023 Apr 1;18(1):58. doi: 10.1186/s11671-023-03842-4. eCollection 2023 Dec. Discov Nano. 2023. PMID: 37032711 Free PMC article. Review.