A link between RNA metabolism and silencing affecting Arabidopsis development - PubMed (original) (raw)
A link between RNA metabolism and silencing affecting Arabidopsis development
Brian D Gregory et al. Dev Cell. 2008 Jun.
Free article
Abstract
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are abundant endogenous small RNAs (smRNAs) that control transcript expression through posttranscriptional gene silencing. Here, we show that concomitant loss of XRN4/EIN5, a 5'-3' exoribonuclease, and ABH1/CBP80, a subunit of the mRNA cap binding complex, results in Arabidopsis plants manifesting myriad developmental defects. We find that ABH1/CBP80 is necessary to obtain proper mature miRNA levels, which suggests this protein affects the miRNA-mediated RNA silencing pathway. Additionally, we show that XRN4/EIN5 affects the levels of a smRNA class that is processed from both sense and antisense strands of approximately 130 endogenous transcripts that apparently are converted to double-stranded RNA (dsRNA) and subsequently processed. We find that the parent transcripts of these smRNAs accumulate in an uncapped form upon loss of XRN4/EIN5, which suggests that uncapped endogenous transcripts can become smRNA biogenesis substrates. Overall, our results reveal unexpected connections between RNA metabolism and silencing pathways.
Comment in
- A silencing safeguard: links between RNA silencing and mRNA processing in Arabidopsis.
Chen X. Chen X. Dev Cell. 2008 Jun;14(6):811-2. doi: 10.1016/j.devcel.2008.05.012. Dev Cell. 2008. PMID: 18539108 Free PMC article.
Similar articles
- A silencing safeguard: links between RNA silencing and mRNA processing in Arabidopsis.
Chen X. Chen X. Dev Cell. 2008 Jun;14(6):811-2. doi: 10.1016/j.devcel.2008.05.012. Dev Cell. 2008. PMID: 18539108 Free PMC article. - Biochemical dissection of RNA silencing in plants.
Tang G, Zamore PD. Tang G, et al. Methods Mol Biol. 2004;257:223-44. doi: 10.1385/1-59259-750-5:223. Methods Mol Biol. 2004. PMID: 14770009 - Biochemical specialization within Arabidopsis RNA silencing pathways.
Qi Y, Denli AM, Hannon GJ. Qi Y, et al. Mol Cell. 2005 Aug 5;19(3):421-8. doi: 10.1016/j.molcel.2005.06.014. Mol Cell. 2005. PMID: 16061187 - MicroRNA-dependent trans-acting siRNA production.
Vaucheret H. Vaucheret H. Sci STKE. 2005 Sep 6;2005(300):pe43. doi: 10.1126/stke.3002005pe43. Sci STKE. 2005. PMID: 16145017 Review. - [Trans-acting short interfering RNAs].
Warkocki Z, Figlerowicz M. Warkocki Z, et al. Postepy Biochem. 2006;52(3):253-9. Postepy Biochem. 2006. PMID: 17201060 Review. Polish.
Cited by
- DNE1 scissorhands: How the power of omics sheds light on the control of mRNA decay.
Raffeiner M. Raffeiner M. Plant Cell. 2024 Sep 3;36(9):3326-3327. doi: 10.1093/plcell/koae219. Plant Cell. 2024. PMID: 39046008 Free PMC article. No abstract available. - Multi-transcriptomics identifies targets of the endoribonuclease DNE1 and highlights its coordination with decapping.
Pouclet A, Pflieger D, Merret R, Carpentier MC, Schiaffini M, Zuber H, Gagliardi D, Garcia D. Pouclet A, et al. Plant Cell. 2024 Sep 3;36(9):3674-3688. doi: 10.1093/plcell/koae175. Plant Cell. 2024. PMID: 38869231 - The spliceosome-associated protein CWC15 promotes miRNA biogenesis in Arabidopsis.
Zhou B, Yu H, Xue Y, Li M, Zhang C, Yu B. Zhou B, et al. Nat Commun. 2024 Mar 16;15(1):2399. doi: 10.1038/s41467-024-46676-z. Nat Commun. 2024. PMID: 38493158 Free PMC article. - PELOTA and HBS1 suppress co-translational messenger RNA decay in Arabidopsis.
Guo R, Gregory BD. Guo R, et al. Plant Direct. 2023 Dec 26;7(12):e553. doi: 10.1002/pld3.553. eCollection 2023 Dec. Plant Direct. 2023. PMID: 38149303 Free PMC article. - Role of miRNAs in sucrose stress response, reactive oxygen species, and anthocyanin biosynthesis in Arabidopsis thaliana.
Azad MF, Dawar P, Esim N, Rock CD. Azad MF, et al. Front Plant Sci. 2023 Nov 3;14:1278320. doi: 10.3389/fpls.2023.1278320. eCollection 2023. Front Plant Sci. 2023. PMID: 38023835 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous