Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human gamma-globin genes - PubMed (original) (raw)

Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human gamma-globin genes

G Zitnik et al. Proc Natl Acad Sci U S A. 1991.

Abstract

We introduced a normal chromosome 11 into GM979 murine erythroleukemia cells by fusing them with Epstein-Barr virus-transformed lymphocytes from a normal individual. In contrast to previous data obtained with other murine erythroleukemia cells, we detected activation of human chromosomal gamma-globin genes in GM979 cells. GM979, unlike previously used murine erythroleukemia cell lines, expresses murine embryonic globin in addition to adult globin. While all the hybrids expressed gamma- and beta-globin, they displayed a wide range of gamma-globin expression in relation to that of beta-globin. No correlation, however, was found in quantitative expression between murine embryonic globin and human gamma-globin in these hybrids, suggesting that the two globins are regulated independently, at least in this cell line. These data indicate that gamma-globin genes from normal, nonerythroid chromosomes are not irreversibly silenced, and they can be activated by a positive trans factor(s) present in GM979 cells.

PubMed Disclaimer

Similar articles

• Adult and fetal human globin genes are expressed following chromosomal transfer into MEL cells.
  Papayannopoulou TH, Lindsley D, Kurachi S, Lewison K, Hemenway T, Melis M, Anagnou NP, Najfeld V. Papayannopoulou TH, et al. Proc Natl Acad Sci U S A. 1985 Feb;82(3):780-4. doi: 10.1073/pnas.82.3.780. Proc Natl Acad Sci U S A. 1985. PMID: 2579380 Free PMC article.
• A chromosome 11-linked determinant controls fetal globin expression and the fetal-to-adult globin switch.
  Melis M, Demopulos G, Najfeld V, Zhang JW, Brice M, Papayannopoulou T, Stamatoyannopoulos G. Melis M, et al. Proc Natl Acad Sci U S A. 1987 Nov;84(22):8105-9. doi: 10.1073/pnas.84.22.8105. Proc Natl Acad Sci U S A. 1987. PMID: 3479779 Free PMC article.
• Developmental regulation of fetal to adult globin gene switching in human fetal erythroid x mouse erythroleukemia cell hybrids.
  Enver T, Brice M, Karlinsey J, Stamatoyannopoulos G, Papayannopoulou T. Enver T, et al. Dev Biol. 1991 Nov;148(1):129-37. doi: 10.1016/0012-1606(91)90323-u. Dev Biol. 1991. PMID: 1936553
• Activation of phenotypic expression of human globin genes from nonerythroid cells by chromosome-dependent transfer to tetraploid mouse erythroleukemia cells.
  Deisseroth A, Hendrick D. Deisseroth A, et al. Proc Natl Acad Sci U S A. 1979 May;76(5):2185-9. doi: 10.1073/pnas.76.5.2185. Proc Natl Acad Sci U S A. 1979. PMID: 287056 Free PMC article.
• Regulation of adult and embryonic genes in human leukemia cells.
  Deisseroth A, Lyding J. Deisseroth A, et al. Am J Hematol. 1987 Dec;26(4):357-64. doi: 10.1002/ajh.2830260409. Am J Hematol. 1987. PMID: 3318411 Review.

Cited by

• Sox6 directly silences epsilon globin expression in definitive erythropoiesis.
  Yi Z, Cohen-Barak O, Hagiwara N, Kingsley PD, Fuchs DA, Erickson DT, Epner EM, Palis J, Brilliant MH. Yi Z, et al. PLoS Genet. 2006 Feb;2(2):e14. doi: 10.1371/journal.pgen.0020014. Epub 2006 Feb 3. PLoS Genet. 2006. PMID: 16462943 Free PMC article.
• Control of globin gene expression during development and erythroid differentiation.
  Stamatoyannopoulos G. Stamatoyannopoulos G. Exp Hematol. 2005 Mar;33(3):259-71. doi: 10.1016/j.exphem.2004.11.007. Exp Hematol. 2005. PMID: 15730849 Free PMC article. Review.
• Induction of human gamma globin gene expression by histone deacetylase inhibitors.
  Cao H, Stamatoyannopoulos G, Jung M. Cao H, et al. Blood. 2004 Jan 15;103(2):701-9. doi: 10.1182/blood-2003-02-0478. Epub 2003 Aug 14. Blood. 2004. PMID: 12920038 Free PMC article.
• Long-distance control of origin choice and replication timing in the human beta-globin locus are independent of the locus control region.
  Cimbora DM, Schübeler D, Reik A, Hamilton J, Francastel C, Epner EM, Groudine M. Cimbora DM, et al. Mol Cell Biol. 2000 Aug;20(15):5581-91. doi: 10.1128/MCB.20.15.5581-5591.2000. Mol Cell Biol. 2000. PMID: 10891496 Free PMC article.
• The locus control region is necessary for gene expression in the human beta-globin locus but not the maintenance of an open chromatin structure in erythroid cells.
  Reik A, Telling A, Zitnik G, Cimbora D, Epner E, Groudine M. Reik A, et al. Mol Cell Biol. 1998 Oct;18(10):5992-6000. doi: 10.1128/MCB.18.10.5992. Mol Cell Biol. 1998. PMID: 9742116 Free PMC article.

References

1. 1. Genes Dev. 1990 Oct;4(10):1650-62 - PubMed
2. 1. Blood. 1983 Mar;61(3):530-9 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7033-7 - PubMed
4. 1. Mol Cell Biol. 1988 Apr;8(4):1725-35 - PubMed
5. 1. Cell. 1983 Oct;34(3):865-79 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central