A staphylococcal GGDEF domain protein regulates biofilm formation independently of cyclic dimeric GMP - PubMed (original) (raw)

FIG. 1.

(A) Alignment of the GGDEF (DUF1) domains from the GdpS and GdpP proteins from S. epidermidis and S. aureus with a consensus (65%) sequence derived from >8,000 GGDEF domains (SMART database). The most conserved residues are capitalized and bolded; the deviations from the invariable residues of the consensus are shown in white on the black background; the deviations from less conserved consensus residues are shown on a gray background. Abbreviations used in the consensus line: •, any; l, aliphatic (I, L, or V); a, aromatic (F, H, W, or Y); c, charged (D, E, H, K, or R); h, hydrophobic (A, C, F, G, H, I, K, L, M, R, T, V, W, or Y); −, negative (D or E); p, polar (C, D, E, H, K, N, Q, R, S, or T); +, positive (H, K, or R); s, small (A, C, D, G, N, P, S, T, or V); u, tiny (A, G, or S); t, turn-like (A, C, D, E, G, H, K, N, Q, R, S, or T). (B) Chromosomal organization of the gdpS (SE0528) and adjacent genes in S. epidermidis. Allele replacement was used to construct a gdpS::Emr mutant in which the gdpS gene is disrupted by the ermB gene inserted at a BsgI restriction enzyme site 401 bp from the start codon. (C) Predicted domain organization of the GdpS protein and its mutant derivatives. TM, predicted transmembrane domain; 5TMR-LYT, conserved transmembrane domain of the LytS-YhcK type.