Pancreatic acinar-to-beta cell transdifferentiation in vitro - PubMed (original) (raw)

Review

doi: 10.2741/3119.

Affiliations

• PMID: 18508625
• DOI: 10.2741/3119

Free article

Review

Pancreatic acinar-to-beta cell transdifferentiation in vitro

Kohtaro Minami et al. Front Biosci. 2008.

Free article

Abstract

Although accumulating evidence indicates that proliferation of pre-existing beta-cells is the major mechanism of the maintenance of postnatal beta-cell mass, new beta-cells can be generated from non-beta-cells under certain conditions in vitro. We have recently shown directly by Cre/loxP-based cell lineage tracing that adult mouse pancreatic acinar cells can be transdifferentiated into insulin-secreting cells in vitro. These newly made cells secrete insulin in response to glucose and other secretagogues, but their secretory capacity is still low compared to that of native beta-cells. To improve the efficiency of generation of insulin-secreting cells from non-beta cells, it is critical to understand the molecular mechanism of such transdifferentiation. Since pancreatic acinar cells are the most abundant cell type in the pancreas, their utilization as a source of surrogate beta-cells is an intriguing approach to cell replacement therapy for type 1 diabetes. This review focuses on current knowledge of the regeneration of pancreatic beta-cells and transdifferentiation of pancreatic acinar-cells into insulin-secreting cells.

PubMed Disclaimer

Similar articles

• Generation of insulin-secreting cells from pancreatic acinar cells of animal models of type 1 diabetes.
  Okuno M, Minami K, Okumachi A, Miyawaki K, Yokoi N, Toyokuni S, Seino S. Okuno M, et al. Am J Physiol Endocrinol Metab. 2007 Jan;292(1):E158-65. doi: 10.1152/ajpendo.00180.2006. Epub 2006 Aug 22. Am J Physiol Endocrinol Metab. 2007. PMID: 16926384
• [Regeneration of the pancreas].
  Minami K, Seino S. Minami K, et al. Nihon Rinsho. 2008 May;66(5):926-31. Nihon Rinsho. 2008. PMID: 18464512 Review. Japanese.
• Acinar phenotype is preserved in human exocrine pancreas cells cultured at low temperature: implications for lineage-tracing of β-cell neogenesis.
  Mfopou JK, Houbracken I, Wauters E, Mathijs I, Song I, Himpe E, Baldan J, Heimberg H, Bouwens L. Mfopou JK, et al. Biosci Rep. 2016 May 6;36(3):e00329. doi: 10.1042/BSR20150259. Print 2016 Jun. Biosci Rep. 2016. PMID: 26987985 Free PMC article.
• Lineage tracing and characterization of insulin-secreting cells generated from adult pancreatic acinar cells.
  Minami K, Okuno M, Miyawaki K, Okumachi A, Ishizaki K, Oyama K, Kawaguchi M, Ishizuka N, Iwanaga T, Seino S. Minami K, et al. Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15116-21. doi: 10.1073/pnas.0507567102. Epub 2005 Oct 6. Proc Natl Acad Sci U S A. 2005. PMID: 16210247 Free PMC article.
• Debates in Pancreatic Beta Cell Biology: Proliferation Versus Progenitor Differentiation and Transdifferentiation in Restoring β Cell Mass.
  Spears E, Serafimidis I, Powers AC, Gavalas A. Spears E, et al. Front Endocrinol (Lausanne). 2021 Aug 6;12:722250. doi: 10.3389/fendo.2021.722250. eCollection 2021. Front Endocrinol (Lausanne). 2021. PMID: 34421829 Free PMC article. Review.

Cited by

• Three-Dimensional Analysis of the Human Pancreas.
  Fowler JL, Lee SS, Wesner ZC, Olehnik SK, Kron SJ, Hara M. Fowler JL, et al. Endocrinology. 2018 Mar 1;159(3):1393-1400. doi: 10.1210/en.2017-03076. Endocrinology. 2018. PMID: 29390052 Free PMC article.
• Stereological analyses of the whole human pancreas.
  Poudel A, Fowler JL, Zielinski MC, Kilimnik G, Hara M. Poudel A, et al. Sci Rep. 2016 Sep 23;6:34049. doi: 10.1038/srep34049. Sci Rep. 2016. PMID: 27658965 Free PMC article.
• Pancreatic β Cell Mass Death.
  Marrif HI, Al-Sunousi SI. Marrif HI, et al. Front Pharmacol. 2016 Apr 6;7:83. doi: 10.3389/fphar.2016.00083. eCollection 2016. Front Pharmacol. 2016. PMID: 27092078 Free PMC article. Review.
• Notch Signaling in Pancreatic Development.
  Li XY, Zhai WJ, Teng CB. Li XY, et al. Int J Mol Sci. 2015 Dec 30;17(1):48. doi: 10.3390/ijms17010048. Int J Mol Sci. 2015. PMID: 26729103 Free PMC article. Review.
• Delta Cell Hyperplasia in Adult Goto-Kakizaki (GK/MolTac) Diabetic Rats.
  Alán L, Olejár T, Cahová M, Zelenka J, Berková Z, Smětáková M, Saudek F, Matěj R, Ježek P. Alán L, et al. J Diabetes Res. 2015;2015:385395. doi: 10.1155/2015/385395. Epub 2015 Jul 6. J Diabetes Res. 2015. PMID: 26236746 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • CORE