Functional domains of the poliovirus receptor - PubMed (original) (raw)

Functional domains of the poliovirus receptor

S Koike et al. Proc Natl Acad Sci U S A. 1991.

Abstract

A number of mutant cDNAs of the human poliovirus receptor were constructed to identify essential regions of the molecule as the receptor. All mutant cDNAs carrying the sequence coding for the entire N-terminal immunoglobulin-like domain (domain I) confer permissiveness for poliovirus to mouse L cells, but a mutant cDNA lacking the sequence for domain I does not. The transformants permissive for poliovirus were able to bind the virus and were also recognized by monoclonal antibody D171, which competes with poliovirus for the cellular receptor. These results strongly suggest that the poliovirus binding site resides in domain I of the receptor. Mutant cDNAs for the sequence encoding the intracellular peptide were also constructed and expressed in mouse L cells. Susceptibility of these cells to poliovirus revealed that the entire putative cytoplasmic domain is not essential for virus infection. Thus, the cytoplasmic domain of the molecule appears not to play a role in the penetration of poliovirus.

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References

1. J Exp Med. 1959 Jul 1;110(1):65-80 - PubMed
1. Biochem J. 1973 Jul;133(3):529-39 - PubMed
1. Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):951-5 - PubMed
1. Cell. 1990 Oct 19;63(2):353-62 - PubMed
1. EMBO J. 1990 Oct;9(10):3217-24 - PubMed

Functional domains of the poliovirus receptor - PubMed (original) (raw)