Crystal structure of the ligand-free G-protein-coupled receptor opsin - PubMed (original) (raw)
. 2008 Jul 10;454(7201):183-7.
doi: 10.1038/nature07063. Epub 2008 Jun 18.
Affiliations
- PMID: 18563085
- DOI: 10.1038/nature07063
Crystal structure of the ligand-free G-protein-coupled receptor opsin
Jung Hee Park et al. Nature. 2008.
Abstract
In the G-protein-coupled receptor (GPCR) rhodopsin, the inactivating ligand 11-cis-retinal is bound in the seven-transmembrane helix (TM) bundle and is cis/trans isomerized by light to form active metarhodopsin II. With metarhodopsin II decay, all-trans-retinal is released, and opsin is reloaded with new 11-cis-retinal. Here we present the crystal structure of ligand-free native opsin from bovine retinal rod cells at 2.9 ångström (A) resolution. Compared to rhodopsin, opsin shows prominent structural changes in the conserved E(D)RY and NPxxY(x)(5,6)F regions and in TM5-TM7. At the cytoplasmic side, TM6 is tilted outwards by 6-7 A, whereas the helix structure of TM5 is more elongated and close to TM6. These structural changes, some of which were attributed to an active GPCR state, reorganize the empty retinal-binding pocket to disclose two openings that may serve the entry and exit of retinal. The opsin structure sheds new light on ligand binding to GPCRs and on GPCR activation.
Similar articles
- Agonist-induced conformational changes in bovine rhodopsin: insight into activation of G-protein-coupled receptors.
Bhattacharya S, Hall SE, Vaidehi N. Bhattacharya S, et al. J Mol Biol. 2008 Oct 3;382(2):539-55. doi: 10.1016/j.jmb.2008.06.084. Epub 2008 Jul 7. J Mol Biol. 2008. PMID: 18638482 - Crystal structure of opsin in its G-protein-interacting conformation.
Scheerer P, Park JH, Hildebrand PW, Kim YJ, Krauss N, Choe HW, Hofmann KP, Ernst OP. Scheerer P, et al. Nature. 2008 Sep 25;455(7212):497-502. doi: 10.1038/nature07330. Nature. 2008. PMID: 18818650 - A G protein-coupled receptor at work: the rhodopsin model.
Hofmann KP, Scheerer P, Hildebrand PW, Choe HW, Park JH, Heck M, Ernst OP. Hofmann KP, et al. Trends Biochem Sci. 2009 Nov;34(11):540-52. doi: 10.1016/j.tibs.2009.07.005. Epub 2009 Oct 21. Trends Biochem Sci. 2009. PMID: 19836958 Review. - Retinal release from opsin in molecular dynamics simulations.
Wang T, Duan Y. Wang T, et al. J Mol Recognit. 2011 Mar-Apr;24(2):350-8. doi: 10.1002/jmr.1087. Epub 2010 Nov 23. J Mol Recognit. 2011. PMID: 21360617 - Multiple switches in G protein-coupled receptor activation.
Ahuja S, Smith SO. Ahuja S, et al. Trends Pharmacol Sci. 2009 Sep;30(9):494-502. doi: 10.1016/j.tips.2009.06.003. Epub 2009 Sep 3. Trends Pharmacol Sci. 2009. PMID: 19732972 Review.
Cited by
- Structural insights into the mechanism and dynamics of proteorhodopsin biogenesis and retinal scavenging.
Hirschi S, Lemmin T, Ayoub N, Kalbermatter D, Pellegata D, Ucurum Z, Gertsch J, Fotiadis D. Hirschi S, et al. Nat Commun. 2024 Aug 13;15(1):6950. doi: 10.1038/s41467-024-50960-3. Nat Commun. 2024. PMID: 39138159 Free PMC article. - Ligand-Free Signaling of G-Protein-Coupled Receptors: Physiology, Pharmacology, and Genetics.
Sadee W. Sadee W. Molecules. 2023 Aug 31;28(17):6375. doi: 10.3390/molecules28176375. Molecules. 2023. PMID: 37687205 Free PMC article. Review. - A short story on how chromophore is hydrolyzed from rhodopsin for recycling.
Hong JD, Palczewski K. Hong JD, et al. Bioessays. 2023 Sep;45(9):e2300068. doi: 10.1002/bies.202300068. Epub 2023 Jul 16. Bioessays. 2023. PMID: 37454357 Free PMC article. Review. - Increasing complexity of opsin expression across stomatopod development.
Palecanda S, Steck M, Porter ML. Palecanda S, et al. Ecol Evol. 2023 May 26;13(5):e10121. doi: 10.1002/ece3.10121. eCollection 2023 May. Ecol Evol. 2023. PMID: 37250447 Free PMC article. - Interdisciplinary biophysical studies of membrane proteins bacteriorhodopsin and rhodopsin.
Fahmy K, Sakmar TP. Fahmy K, et al. Biophys Rev. 2022 Oct 8;15(1):111-125. doi: 10.1007/s12551-022-01003-y. eCollection 2023 Feb. Biophys Rev. 2022. PMID: 36909961 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases