In vivo nanosecond laser axotomy: cavitation dynamics and vesicle transport - PubMed (original) (raw)
. 2008 Jun 23;16(13):9884-94.
doi: 10.1364/oe.16.009884.
Affiliations
- PMID: 18575558
- DOI: 10.1364/oe.16.009884
Free article
In vivo nanosecond laser axotomy: cavitation dynamics and vesicle transport
G Nageswara Rao et al. Opt Express. 2008.
Free article
Abstract
Nanosecond laser pulses (lambda = 355 nm) were used to cut mechanosensory neurons in Caenorhabditis elegans and motorneurons in Drosophila melanogaster larvae. A pulse energy range of 0.8-1.2 microJ and < 20 pulses in single shot mode were sufficient to generate axonal cuts. Viability post-surgery was >95% for C. elegans and 60% for Drosophila. Cavitation bubble dynamics generated due to laser-induced plasma formation were observed in vivo by time-resolved imaging in both organisms. Bubble oscillations were severely damped in vivo and cavitation dynamics were complete within 100 ns in C. elegans and 800 ns in Drosophila. We report the use of this system to study axonal transport for the first time and discuss advantages of nanosecond lasers compared to femtosecond sources for such procedures.
Similar articles
- Construction of a femtosecond laser microsurgery system.
Steinmeyer JD, Gilleland CL, Pardo-Martin C, Angel M, Rohde CB, Scott MA, Yanik MF. Steinmeyer JD, et al. Nat Protoc. 2010 Mar;5(3):395-407. doi: 10.1038/nprot.2010.4. Epub 2010 Feb 11. Nat Protoc. 2010. PMID: 20203659 Free PMC article. - Real time imaging of femtosecond laser induced nano-neurosurgery dynamics in C. elegans.
Santos SI, Mathew M, Loza-Alvarez P. Santos SI, et al. Opt Express. 2010 Jan 4;18(1):364-77. doi: 10.1364/OE.18.000364. Opt Express. 2010. PMID: 20173856 - Time-resolved observations of shock waves and cavitation bubbles generated by femtosecond laser pulses in corneal tissue and water.
Juhasz T, Kastis GA, Suárez C, Bor Z, Bron WE. Juhasz T, et al. Lasers Surg Med. 1996;19(1):23-31. doi: 10.1002/(SICI)1096-9101(1996)19:1<23::AID-LSM4>3.0.CO;2-S. Lasers Surg Med. 1996. PMID: 8836993 - Laser microsurgery in Caenorhabditis elegans.
Fang-Yen C, Gabel CV, Samuel AD, Bargmann CI, Avery L. Fang-Yen C, et al. Methods Cell Biol. 2012;107:177-206. doi: 10.1016/B978-0-12-394620-1.00006-0. Methods Cell Biol. 2012. PMID: 22226524 Free PMC article. Review. - Application of femtosecond-laser induced nanostructures in optical memory.
Shimotsuma Y, Sakakura M, Miura K, Qiu J, Kazansky PG, Fujita K, Hirao K. Shimotsuma Y, et al. J Nanosci Nanotechnol. 2007 Jan;7(1):94-104. J Nanosci Nanotechnol. 2007. PMID: 17455477 Review.
Cited by
- Axon regeneration in C. elegans: Worming our way to mechanisms of axon regeneration.
Byrne AB, Hammarlund M. Byrne AB, et al. Exp Neurol. 2017 Jan;287(Pt 3):300-309. doi: 10.1016/j.expneurol.2016.08.015. Epub 2016 Aug 26. Exp Neurol. 2017. PMID: 27569538 Free PMC article. Review. - F-box protein FBXB-65 regulates anterograde transport of the kinesin-3 motor UNC-104 through a PTM near its cargo-binding PH domain.
Sabharwal V, Boyanapalli SPP, Shee A, Nonet ML, Nandi A, Chaudhuri D, Koushika SP. Sabharwal V, et al. J Cell Sci. 2024 Apr 1;137(7):jcs261553. doi: 10.1242/jcs.261553. Epub 2024 Apr 16. J Cell Sci. 2024. PMID: 38477340 - Axon regeneration in C. elegans.
Hammarlund M, Jin Y. Hammarlund M, et al. Curr Opin Neurobiol. 2014 Aug;27:199-207. doi: 10.1016/j.conb.2014.04.001. Epub 2014 May 4. Curr Opin Neurobiol. 2014. PMID: 24794753 Free PMC article. Review. - In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons.
Dey S, Ghosh-Roy A. Dey S, et al. J Vis Exp. 2021 Nov 20;(177):10.3791/62744. doi: 10.3791/62744. J Vis Exp. 2021. PMID: 34866634 Free PMC article. - Constructing a low-budget laser axotomy system to study axon regeneration in C. elegans.
Williams W, Nix P, Bastiani M. Williams W, et al. J Vis Exp. 2011 Nov 15;(57):3331. doi: 10.3791/3331. J Vis Exp. 2011. PMID: 22126922 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous