Induced expression of syndecan in healing wounds - PubMed (original) (raw)
Induced expression of syndecan in healing wounds
K Elenius et al. J Cell Biol. 1991 Aug.
Abstract
We have studied the expression of an integral cell surface proteoglycan, syndecan, during the healing of cutaneous wounds, using immunohistochemical and in situ hybridization methods. In normal mouse skin, both syndecan antigen and mRNA were found to be expressed exclusively by epidermal and hair follicle cells. After incision and subsequent suturing, remarkably increased amounts of syndecan on the cell surfaces of migrating and proliferating epidermal cells and on hair follicle cells adjacent to wound margins were noted. This increased syndecan expression was shown to be a consequence of greater amounts of syndecan mRNA. Induction was observed already 1 d after wounding, was most significant at the time of intense cell proliferation, and was still observable 14 d after incision. The migrating cells of the leading edge of the epithelium also showed enhanced syndecan expression, although clearly less than that seen in the proliferating epithelium. The merging epithelial cells at the site of incision showed little or no syndecan expression; increased syndecan expression, however, was detected during later epithelial stratification. When wounds were left unsutured, in situ hybridization experiments also revealed scattered syndecan-positive signals in the granulation tissue near the migrating epidermal sheet. By immunohistochemical analysis, positive staining in granulation tissue was observed around vascular endothelial cells in a subpopulation of growing capillaries. Induction of syndecan in granulation tissue both at the protein and mRNA levels was temporally and spatially highly restricted. Granulation tissue, which formed in viscose cellulose sponge cylinders placed under the skin of rats, was also found to produce 3.4 and 2.6 kb mRNA species of syndecan similar to that observed in the normal murine mammary epithelial cell line, NMuMG. These results suggest that syndecan may have a unique and important role as a cell adhesion and a growth factor-binding molecule not only during embryogenesis but also during tissue regeneration in mature tissues.
Similar articles
- Suppression of syndecan-1 expression in endothelial cells by tumor necrosis factor-alpha.
Kainulainen V, Nelimarkka L, Järveläinen H, Laato M, Jalkanen M, Elenius K. Kainulainen V, et al. J Biol Chem. 1996 Aug 2;271(31):18759-66. doi: 10.1074/jbc.271.31.18759. J Biol Chem. 1996. PMID: 8702532 - Different mechanisms of syndecan-1 activation through a fibroblast-growth-factor-inducible response element (FiRE) in mucosal and cutaneous wounds.
Rautava J, Soukka T, Heikinheimo K, Miettinen PJ, Happonen RP, Jaakkola P. Rautava J, et al. J Dent Res. 2003 May;82(5):382-7. doi: 10.1177/154405910308200511. J Dent Res. 2003. PMID: 12709506 - Expression of syndecan gene is induced early, is transient, and correlates with changes in mesenchymal cell proliferation during tooth organogenesis.
Vainio S, Jalkanen M, Vaahtokari A, Sahlberg C, Mali M, Bernfield M, Thesleff I. Vainio S, et al. Dev Biol. 1991 Oct;147(2):322-33. doi: 10.1016/0012-1606(91)90290-j. Dev Biol. 1991. PMID: 1717321 - Transcriptional regulation of Syndecan-1 expression by growth factors.
Jaakkola P, Jalkanen M. Jaakkola P, et al. Prog Nucleic Acid Res Mol Biol. 1999;63:109-38. doi: 10.1016/s0079-6603(08)60721-7. Prog Nucleic Acid Res Mol Biol. 1999. PMID: 10506830 Review. - Syndecan, a developmentally regulated cell surface proteoglycan that binds extracellular matrix and growth factors.
Bernfield M, Sanderson RD. Bernfield M, et al. Philos Trans R Soc Lond B Biol Sci. 1990 Mar 12;327(1239):171-86. doi: 10.1098/rstb.1990.0052. Philos Trans R Soc Lond B Biol Sci. 1990. PMID: 1969657 Review.
Cited by
- A view of the genetic and proteomic profile of extracellular matrix molecules in aging and stroke.
Chmelova M, Androvic P, Kirdajova D, Tureckova J, Kriska J, Valihrach L, Anderova M, Vargova L. Chmelova M, et al. Front Cell Neurosci. 2023 Nov 30;17:1296455. doi: 10.3389/fncel.2023.1296455. eCollection 2023. Front Cell Neurosci. 2023. PMID: 38107409 Free PMC article. - Altered Distribution and Expression of Syndecan-1 and -4 as an Additional Hallmark in Psoriasis.
Koliakou E, Eleni MM, Koumentakou I, Bikiaris N, Konstantinidou P, Rousselle P, Anestakis D, Lazaridou E, Kalloniati E, Miliaras D, Michopoulou A. Koliakou E, et al. Int J Mol Sci. 2022 Jun 10;23(12):6511. doi: 10.3390/ijms23126511. Int J Mol Sci. 2022. PMID: 35742957 Free PMC article. - CD39 and CD326 Are Bona Fide Markers of Murine and Human Plasma Cells and Identify a Bone Marrow Specific Plasma Cell Subpopulation in Lupus.
Dang VD, Mohr E, Szelinski F, Le TA, Ritter J, Hinnenthal T, Stefanski AL, Schrezenmeier E, Ocvirk S, Hipfl C, Hardt S, Cheng Q, Hiepe F, Löhning M, Dörner T, Lino AC. Dang VD, et al. Front Immunol. 2022 Apr 8;13:873217. doi: 10.3389/fimmu.2022.873217. eCollection 2022. Front Immunol. 2022. PMID: 35464469 Free PMC article. - Thy-1 (CD90), Integrins and Syndecan 4 are Key Regulators of Skin Wound Healing.
Pérez LA, Leyton L, Valdivia A. Pérez LA, et al. Front Cell Dev Biol. 2022 Feb 3;10:810474. doi: 10.3389/fcell.2022.810474. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 35186924 Free PMC article. Review. - Growth factor expression is enhanced, and extracellular matrix proteins are depressed in healing skin wounds in septic patients compared with healthy controls.
Jaurila H, Koskela M, Koivukangas V, Gäddnäs F, Salo T, Ala-Kokko TI. Jaurila H, et al. APMIS. 2022 Mar;130(3):155-168. doi: 10.1111/apm.13175. Epub 2022 Jan 23. APMIS. 2022. PMID: 34939229 Free PMC article.
References
- J Biol Chem. 1990 Oct 15;265(29):17837-43 - PubMed
- Surg Gynecol Obstet. 1971 Dec;133(6):1003-7 - PubMed
- Cell. 1991 Mar 8;64(5):867-9 - PubMed
- Dev Biol. 1988 Oct;129(2):565-72 - PubMed
- Science. 1987 Sep 11;237(4820):1333-6 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources