DNA gyrase: an enzyme that introduces superhelical turns into DNA - PubMed (original) (raw)

DNA gyrase: an enzyme that introduces superhelical turns into DNA

M Gellert et al. Proc Natl Acad Sci U S A. 1976 Nov.

Abstract

Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA.

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References

    1. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1072-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3524-8 - PubMed
    1. J Mol Biol. 1975 Jul 25;96(1):201-5 - PubMed
    1. Biochemistry. 1975 Oct 21;14(21):4634-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2550-4 - PubMed

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