DNA gyrase: an enzyme that introduces superhelical turns into DNA - PubMed (original) (raw)
DNA gyrase: an enzyme that introduces superhelical turns into DNA
M Gellert et al. Proc Natl Acad Sci U S A. 1976 Nov.
Abstract
Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA.
References
- Proc Natl Acad Sci U S A. 1975 Mar;72(3):1072-6 - PubMed
- Proc Natl Acad Sci U S A. 1976 Oct;73(10):3524-8 - PubMed
- J Mol Biol. 1975 Jul 25;96(1):201-5 - PubMed
- Biochemistry. 1975 Oct 21;14(21):4634-8 - PubMed
- Proc Natl Acad Sci U S A. 1975 Jul;72(7):2550-4 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases