Evidence that a downstream pseudoknot is required for translational read-through of the Moloney murine leukemia virus gag stop codon - PubMed (original) (raw)

Evidence that a downstream pseudoknot is required for translational read-through of the Moloney murine leukemia virus gag stop codon

N M Wills et al. Proc Natl Acad Sci U S A. 1991.

Abstract

Approximately 5% of the ribosomes translating the gag gene of murine leukemia viruses read through the UAG terminator and translate the in-frame pol gene to produce the gag-pol fusion polyprotein, the sole source of the pol gene products. We show that a pseudoknot located eight nucleotides 3' of the UAG codon in the Moloney murine leukemia virus is required for read-through. This requirement is markedly different from that known to be involved in other cases of read-through but surprisingly similar to some stimulatory sequences known to promote ribosomal frameshifting.

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References

1. 1. J Virol. 1984 Feb;49(2):471-8 - PubMed
2. 1. Nature. 1981 Oct 15-21;293(5833):543-8 - PubMed
3. 1. J Virol. 1980 May;34(2):464-73 - PubMed
4. 1. Virology. 1978 May 15;86(2):329-43 - PubMed
5. 1. Cell. 1978 Jan;13(1):189-99 - PubMed

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