Immunophenotyping of plasma cells - PubMed (original) (raw)
Immunophenotyping of plasma cells
Andy C Rawstron. Curr Protoc Cytom. 2006 May.
Abstract
Plasma cell enumeration and immunophenotyping has been shown to be of value in predicting the outcome for patients with myeloma. Detection of abnormal plasma cells at a leukocyte level >0.01% at the end of therapy predicts early relapse. Although clonally related populations at varying stages of differentiation are reported to be present in myeloma, only the CD138+ fraction has been shown to directly correlate with the outcome. In addition, the plasma cell immunophenotype in monoclonal gammopathy of undetermined significance (MGUS) is reported to correlate with the risk of progression to myeloma. This protocol provides a four-color flow cytometry method for enumeration and classification of plasma cells in both diagnostic and post-treatment samples. Plasma cells are identified by strong expression of CD38 and CD138. Neoplastic plasma cells are separated from their normal counterparts by aberrant expression of CD19 and other relevant markers in combination with intracellular immunoglobulin light chain restriction.
Similar articles
- CD19-CD45 low/- CD38 high/CD138+ plasma cells enrich for human tumorigenic myeloma cells.
Kim D, Park CY, Medeiros BC, Weissman IL. Kim D, et al. Leukemia. 2012 Dec;26(12):2530-7. doi: 10.1038/leu.2012.140. Epub 2012 May 30. Leukemia. 2012. PMID: 22733078 - Immunophenotype of normal vs. myeloma plasma cells: Toward antibody panel specifications for MRD detection in multiple myeloma.
Flores-Montero J, de Tute R, Paiva B, Perez JJ, Böttcher S, Wind H, Sanoja L, Puig N, Lecrevisse Q, Vidriales MB, van Dongen JJ, Orfao A. Flores-Montero J, et al. Cytometry B Clin Cytom. 2016 Jan;90(1):61-72. doi: 10.1002/cyto.b.21265. Epub 2015 Jul 31. Cytometry B Clin Cytom. 2016. PMID: 26100534 Review. - Review of phenotypic markers used in flow cytometric analysis of MGUS and MM, and applicability of flow cytometry in other plasma cell disorders.
Raja KR, Kovarova L, Hajek R. Raja KR, et al. Br J Haematol. 2010 May;149(3):334-51. doi: 10.1111/j.1365-2141.2010.08121.x. Epub 2010 Mar 1. Br J Haematol. 2010. PMID: 20201947 Review.
Cited by
- Immunophenotype of normal and myelomatous plasma-cell subsets.
Robillard N, Wuillème S, Moreau P, Béné MC. Robillard N, et al. Front Immunol. 2014 Mar 31;5:137. doi: 10.3389/fimmu.2014.00137. eCollection 2014. Front Immunol. 2014. PMID: 24744760 Free PMC article. - Rapid production of antigen-specific monoclonal antibodies from a variety of animals.
Kurosawa N, Yoshioka M, Fujimoto R, Yamagishi F, Isobe M. Kurosawa N, et al. BMC Biol. 2012 Sep 28;10:80. doi: 10.1186/1741-7007-10-80. BMC Biol. 2012. PMID: 23017270 Free PMC article. - Standardizing immunophenotyping for the Human Immunology Project.
Maecker HT, McCoy JP, Nussenblatt R. Maecker HT, et al. Nat Rev Immunol. 2012 Feb 17;12(3):191-200. doi: 10.1038/nri3158. Nat Rev Immunol. 2012. PMID: 22343568 Free PMC article. Review. - Isolation of Human CD138(+) Microparticles from the Plasma of Patients with Multiple Myeloma.
Krishnan SR, Luk F, Brown RD, Suen H, Kwan Y, Bebawy M. Krishnan SR, et al. Neoplasia. 2016 Jan;18(1):25-32. doi: 10.1016/j.neo.2015.11.011. Neoplasia. 2016. PMID: 26806349 Free PMC article. - SEC-seq: association of molecular signatures with antibody secretion in thousands of single human plasma cells.
Cheng RY, de Rutte J, Ito CEK, Ott AR, Bosler L, Kuo WY, Liang J, Hall BE, Rawlings DJ, Di Carlo D, James RG. Cheng RY, et al. Nat Commun. 2023 Jun 15;14(1):3567. doi: 10.1038/s41467-023-39367-8. Nat Commun. 2023. PMID: 37322036 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials