Boosting tandem affinity purification of plant protein complexes - PubMed (original) (raw)

Boosting tandem affinity purification of plant protein complexes

Jelle Van Leene et al. Trends Plant Sci. 2008 Oct.

Abstract

Protein-interaction mapping based on the tandem affinity purification (TAP) approach has been successfully established for several systems, such as yeast and mammalian cells. However, relatively few protein complex purifications have been reported for plants. Here, we highlight solutions for the pitfalls and propose a major breakthrough in the quest for a better TAP tag in plants.

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