Characterization of ADAR1-mediated modulation of gene expression - PubMed (original) (raw)
. 2008 Dec 5;377(1):170-5.
doi: 10.1016/j.bbrc.2008.09.109. Epub 2008 Oct 7.
Affiliations
- PMID: 18835380
- DOI: 10.1016/j.bbrc.2008.09.109
Characterization of ADAR1-mediated modulation of gene expression
Willemijn M Gommans et al. Biochem Biophys Res Commun. 2008.
Abstract
Conversion of adenosine into inosine in RNA molecules constitutes an important post-transcriptional mechanism for generating transcript diversity and is catalyzed by adenosine deaminases acting on RNA (ADARs). Intriguingly, we observed that the editing enzyme ADAR1 enhances reporter gene expression in a cellular, plasmid-based system. The induction of gene expression is independent of the used reporter transgene or the promoter type, but relies on the RNA editing activity and specificity of ADAR1. More detailed analysis indicates that the effect is due to enhanced reporter gene transcription. Induction of gene expression by ADAR1 is lost when the reporter expression cassette is placed in a chromosomal environment. Our results suggest that a cellular, ADAR1-specific RNA editing substrate causes upregulation of plasmid-based gene expression.
Similar articles
- Modulation of ADAR1 editing activity by Z-RNA in vitro.
Koeris M, Funke L, Shrestha J, Rich A, Maas S. Koeris M, et al. Nucleic Acids Res. 2005 Sep 21;33(16):5362-70. doi: 10.1093/nar/gki849. Print 2005. Nucleic Acids Res. 2005. PMID: 16177183 Free PMC article. - Vaccinia virus E3L interferon resistance protein inhibits the interferon-induced adenosine deaminase A-to-I editing activity.
Liu Y, Wolff KC, Jacobs BL, Samuel CE. Liu Y, et al. Virology. 2001 Oct 25;289(2):378-87. doi: 10.1006/viro.2001.1154. Virology. 2001. PMID: 11689059 - [RNA editing in eukaryotic genome expression].
Pankratova EV, Stepchenko AG. Pankratova EV, et al. Genetika. 2010 Jan;46(1):5-13. Genetika. 2010. PMID: 20198873 Review. Russian. - The large form of ADAR 1 is responsible for enhanced hepatitis delta virus RNA editing in interferon-alpha-stimulated host cells.
Hartwig D, Schütte C, Warnecke J, Dorn I, Hennig H, Kirchner H, Schlenke P. Hartwig D, et al. J Viral Hepat. 2006 Mar;13(3):150-7. doi: 10.1111/j.1365-2893.2005.00663.x. J Viral Hepat. 2006. PMID: 16475990 - A-to-I editing challenger or ally to the microRNA process.
Ohman M. Ohman M. Biochimie. 2007 Oct;89(10):1171-6. doi: 10.1016/j.biochi.2007.06.002. Epub 2007 Jun 8. Biochimie. 2007. PMID: 17628290 Review.
Cited by
- Reporters transiently transfected into mammalian cells are highly sensitive to translational repression induced by dsRNA expression.
Nejepinska J, Malik R, Wagner S, Svoboda P. Nejepinska J, et al. PLoS One. 2014 Jan 27;9(1):e87517. doi: 10.1371/journal.pone.0087517. eCollection 2014. PLoS One. 2014. PMID: 24475301 Free PMC article. - ADAR1 is a new target of METTL3 and plays a pro-oncogenic role in glioblastoma by an editing-independent mechanism.
Tassinari V, Cesarini V, Tomaselli S, Ianniello Z, Silvestris DA, Ginistrelli LC, Martini M, De Angelis B, De Luca G, Vitiani LR, Fatica A, Locatelli F, Gallo A. Tassinari V, et al. Genome Biol. 2021 Jan 28;22(1):51. doi: 10.1186/s13059-021-02271-9. Genome Biol. 2021. PMID: 33509238 Free PMC article. - Adenosine deaminase ADAR1 increases gene expression at the translational level by decreasing protein kinase PKR-dependent eIF-2alpha phosphorylation.
Wang Y, Samuel CE. Wang Y, et al. J Mol Biol. 2009 Nov 6;393(4):777-87. doi: 10.1016/j.jmb.2009.08.070. Epub 2009 Sep 3. J Mol Biol. 2009. PMID: 19733181 Free PMC article. - MicroRNA-mediated loss of ADAR1 in metastatic melanoma promotes tumor growth.
Nemlich Y, Greenberg E, Ortenberg R, Besser MJ, Barshack I, Jacob-Hirsch J, Jacoby E, Eyal E, Rivkin L, Prieto VG, Chakravarti N, Duncan LM, Kallenberg DM, Galun E, Bennett DC, Amariglio N, Bar-Eli M, Schachter J, Rechavi G, Markel G. Nemlich Y, et al. J Clin Invest. 2013 Jun;123(6):2703-18. doi: 10.1172/JCI62980. J Clin Invest. 2013. PMID: 23728176 Free PMC article. - InvAluable junk: the cellular impact and function of Alu and B2 RNAs.
Walters RD, Kugel JF, Goodrich JA. Walters RD, et al. IUBMB Life. 2009 Aug;61(8):831-7. doi: 10.1002/iub.227. IUBMB Life. 2009. PMID: 19621349 Free PMC article. Review.