Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase - PubMed (original) (raw)
. 1991 Sep;5(9):1589-600.
doi: 10.1101/gad.5.9.1589.
Affiliations
- PMID: 1885001
- DOI: 10.1101/gad.5.9.1589
Free article
Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase
S J Brill et al. Genes Dev. 1991 Sep.
Free article
Abstract
Replication factor-A (RF-A) is a three-subunit protein complex originally purified from human cells as an essential component for SV40 DNA replication in vitro. We have previously identified a functionally homologous three-subunit protein complex from the yeast Saccharomyces cerevisiae. Here we report the cloning and characterization of the genes encoding RF-A from S. cerevisiae. Each of the three subunits is encoded by a single essential gene. Cells carrying null mutations in any of the three genes arrest as budded and multiply budded cells. All three genes are expressed in a cell-cycle-dependent manner; the mRNA for each subunit peaks at the G1/S-phase boundary. A comparison of protein sequences indicates that the human p34 subunit is 29% identical to the corresponding RFA2 gene product. However, expression of the human protein fails to rescue the rfa2::TRP1 disruption.
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