Anatomy of herpes simplex virus DNA VII. alpha-RNA is homologous to noncontiguous sites in both the L and S components of viral DNA - PubMed (original) (raw)
Anatomy of herpes simplex virus DNA VII. alpha-RNA is homologous to noncontiguous sites in both the L and S components of viral DNA
P C Jones et al. J Virol. 1977 Jan.
Abstract
Previous reports from this laboratory (Honess and Roizman, 1974) have operationally defined alpha polypeptides as the viral proteins that are synthesized first in HEp-2 cells treated with cycloheximide from the time of infection with herpes simplex virus type 1 until the withdrawal of the drug 12 to 15 h after infection. It has also been shown that the viral RNA (designated alpha RNA) that accumulates in the cytoplasm during cycloheximide treatment and on polyribosomes immediately upon withdrawal of the drug is homologous to 10 to 12% of viral DNA, whereas the viral RNA accumulating in the cytoplasm of untreated cells at 8 to 14 h after infection is homologous to 43% of viral DNA (Kozak and Roizman, 1974). In the present study, alpha RNA and cytoplasmic RNA extracted from untreated cells 8 h after infection were each hybridized in liquid to in vitro labeled restriction endonuclease fragments generated by cleavage of herpes simplex virus type 1 DNA with Hsu I, with Bgl II, and with both enzymes simultaneously. The data show that only a subset of the fragments hybridized to alpha RNA, and these are scattered within both the L and S components of the DNA. There are at least five noncontiguous regions in the DNA homologous to alpha RNA; two of these are located partially within the reiterated sequences in the S component. All fragments tested hybridized more extensively with 8-h cytoplasmic RNA than with alpha RNA. Four adjacent fragments, corresponding to 30% of the DNA and mapping within the L component, hybridized exclusively with the cytoplasmic RNA extracted from cells 8 h after infection.
Similar articles
- RNA synthesis in cells infected with herpes simple virus. XIII. Differences in the methylation patterns of viral RNA during the reproductive cycle.
Bartkoski M, Roizman B. Bartkoski M, et al. J Virol. 1976 Dec;20(3):583-8. doi: 10.1128/JVI.20.3.583-588.1976. J Virol. 1976. PMID: 186635 Free PMC article. - Release of viruses and viral DNA from nucleus to cytoplasm of HeLa cells at late stages of productive adenovirus infection as revealed by electron microscope in situ hybridization.
Puvion-Dutilleul F, Besse S, Pichard E, Cajean-Feroldi C. Puvion-Dutilleul F, et al. Biol Cell. 1998 Jan;90(1):5-38. doi: 10.1016/s0248-4900(98)80230-x. Biol Cell. 1998. PMID: 9691424 Review.
Cited by
- Properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene: mechanisms of reversion to a thymidine kinase-negative phenotype.
Bastow KF, Darby G, Wildy P, Minson AC. Bastow KF, et al. J Virol. 1980 Dec;36(3):746-55. doi: 10.1128/JVI.36.3.746-755.1980. J Virol. 1980. PMID: 16789205 Free PMC article. - The ICP22 protein of equine herpesvirus 1 cooperates with the IE protein to regulate viral gene expression.
Kim SK, Holden VR, O'Callaghan DJ. Kim SK, et al. J Virol. 1997 Feb;71(2):1004-12. doi: 10.1128/JVI.71.2.1004-1012.1997. J Virol. 1997. PMID: 8995619 Free PMC article. - Swine testis cells contain functional heparan sulfate but are defective in entry of herpes simplex virus.
Subramanian G, McClain DS, Perez A, Fuller AO. Subramanian G, et al. J Virol. 1994 Sep;68(9):5667-76. doi: 10.1128/JVI.68.9.5667-5676.1994. J Virol. 1994. PMID: 8057447 Free PMC article. - DNA sequence elements required for regulated expression of the HSV-1 glycoprotein D gene lie within 83 bp of the RNA capsites.
Everett RD. Everett RD. Nucleic Acids Res. 1983 Oct 11;11(19):6647-66. doi: 10.1093/nar/11.19.6647. Nucleic Acids Res. 1983. PMID: 6314251 Free PMC article.
References
- J Virol. 1973 Jun;11(6):886-92 - PubMed
- Nature. 1973 Mar 2;242(5392):44-7 - PubMed
- J Virol. 1974 Jul;14(1):8-19 - PubMed
- Proc Natl Acad Sci U S A. 1974 Nov;71(11):4322-6 - PubMed
- Biochemistry. 1972 Feb 15;11(4):637-41 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources