Molecular cloning and functional analysis of three subunits of yeast proteasome - PubMed (original) (raw)

Comparative Study

Molecular cloning and functional analysis of three subunits of yeast proteasome

Y Emori et al. Mol Cell Biol. 1991 Jan.

Abstract

The genes encoding three subunits of Saccharomyces cerevisiae proteasome were cloned and sequenced. The deduced amino acid sequences were homologous not only to each other (30 to 40% identity) but also to those of rat and Drosophila proteasomes (25 to 65% identity). However, none of these sequences showed any similarity to any other known sequences, including various proteases, suggesting that these proteasome subunits may constitute a unique gene family. Gene disruption analyses revealed that two of the three subunits (subunits Y7 and Y8) are essential for growth, indicating that the proteasome and its individual subunits play an indispensable role in fundamental biological processes. On the other hand, subunit Y13 is not essential; haploid cells with a disrupted Y13 gene can proliferate, although the doubling time is longer than that of cells with nondisrupted genes. In addition, biochemical analysis revealed that proteasome prepared from the Y13 disrupted cells contains tryptic and chymotryptic activities equivalent to those of nondisrupted cells, indicating that the Y13 subunit is not essential for tryptic or chymotryptic activity. However, the chymotryptic activity of the Y13 disrupted cells is not dependent on sodium dodecyl sulfate (SDS), an activator of proteasome, since nearly full activity was observed in the absence of SDS. Thus, the activity in proteasome of the Y13 disrupted cells might result in unregulated intracellular proteolysis, thus leading to the prolonged cell cycle. These results indicate that cloned proteasome subunits having similar sequences to the yeast Y13 subunit are structural, but not catalytic, components of proteasome. It is also suggested that two subunits (Y7 and Y8) might occupy positions essential to proteasome structure or activity, whereas subunit Y13 is in a nonessential but important position.

PubMed Disclaimer

Cited by

Proteasomes are regulated by interferon gamma: implications for antigen processing.
Yang Y, Waters JB, Früh K, Peterson PA. Yang Y, et al. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4928-32. doi: 10.1073/pnas.89.11.4928. Proc Natl Acad Sci U S A. 1992. PMID: 1594596 Free PMC article.
The prosomal RNA-binding protein p27K is a member of the alpha-type human prosomal gene family.
Bey F, Silva Pereira I, Coux O, Viegas-Péquignot E, Recillas Targa F, Nothwang HG, Dutrillaux B, Scherrer K. Bey F, et al. Mol Gen Genet. 1993 Feb;237(1-2):193-205. doi: 10.1007/BF00282801. Mol Gen Genet. 1993. PMID: 7681138
Mutations in PRG1, a yeast proteasome-related gene, cause defects in nuclear division and are suppressed by deletion of a mitotic cyclin gene.
Friedman H, Snyder M. Friedman H, et al. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2031-5. doi: 10.1073/pnas.91.6.2031. Proc Natl Acad Sci U S A. 1994. PMID: 8134345 Free PMC article.
Proteasome Control of [URE3] Prion Propagation by Degradation of Anti-Prion Proteins Cur1 and Btn2 in Saccharomyces cerevisiae.
Edskes HK, Stroobant EE, DeWilde MP, Bezsonov EE, Wickner RB. Edskes HK, et al. Genetics. 2021 May 17;218(1):iyab037. doi: 10.1093/genetics/iyab037. Genetics. 2021. PMID: 33742650 Free PMC article.
A genomic screen in yeast reveals novel aspects of nonstop mRNA metabolism.
Wilson MA, Meaux S, van Hoof A. Wilson MA, et al. Genetics. 2007 Oct;177(2):773-84. doi: 10.1534/genetics.107.073205. Epub 2007 Jul 29. Genetics. 2007. PMID: 17660569 Free PMC article.

References

1. Nature. 1988 Jan 14;331(6152):190-2 - PubMed
1. J Biochem. 1986 Sep;100(3):753-63 - PubMed
1. FEBS Lett. 1985 Sep 9;189(1):119-23 - PubMed
1. Biochim Biophys Acta. 1986 Jul 16;882(3):305-10 - PubMed
1. Biochim Biophys Acta. 1986 Jul 16;882(3):297-304 - PubMed

Molecular cloning and functional analysis of three subunits of yeast proteasome - PubMed (original) (raw)