The spo0K locus of Bacillus subtilis is homologous to the oligopeptide permease locus and is required for sporulation and competence - PubMed (original) (raw)

The spo0K locus of Bacillus subtilis is homologous to the oligopeptide permease locus and is required for sporulation and competence

D Z Rudner et al. J Bacteriol. 1991 Feb.

Abstract

Spore formation in Bacillus subtilis is a dramatic response to environmental signals that is controlled in part by a two-component regulatory system composed of a histidine protein kinase (SpoIIJ) and a transcriptional regulator (Spo0A). The spo0K locus plays an important but undefined role in the initiation of sporulation and in the development of genetic competence. spoIIJ spo0K double mutants had a more severe defect in sporulation than either single mutant. Overproduction of the spoIIJ gene product resulted in the suppression of the sporulation defect, but not the competence defect, caused by mutations in the spo0K locus. On the basis of the phenotype of the spoIIJ spo0K double mutant and the effect of overproduction of the spoIIJ gene product, a transposon insertion in the spo0K locus was isolated. The spo0K locus was cloned and sequenced. spo0K proved to be an operon of five genes that is homologous to the oligopeptide permease (opp) operon of Salmonella typhimurium and related to a large family of membrane transport systems. The requirement for the transport system encoded by spo0K in the development of competence was somewhat different than its requirement in the system encoded by spo0K in the development of competence was somewhat different than its requirement in the initiation of sporulation. Disruption of the last open reading frame in the spo0K operon caused a defect in competence but had little or no effect on sporulation. We hypothesize that the transport system encoded by spo0K may have a role in sensing extracellular peptide factors that we have shown are required for efficient sporulation and perhaps in sensing similar factors that may be necessary for genetic competence.

PubMed Disclaimer

Cited by

Genetic competence in Bacillus subtilis.
Dubnau D. Dubnau D. Microbiol Rev. 1991 Sep;55(3):395-424. doi: 10.1128/mr.55.3.395-424.1991. Microbiol Rev. 1991. PMID: 1943994 Free PMC article. Review.
ScoC regulates peptide transport and sporulation initiation in Bacillus subtilis.
Koide A, Perego M, Hoch JA. Koide A, et al. J Bacteriol. 1999 Jul;181(13):4114-7. doi: 10.1128/JB.181.13.4114-4117.1999. J Bacteriol. 1999. PMID: 10383984 Free PMC article.
Increase of sensitivity to aminoglycoside antibiotics by polyamine-induced protein (oligopeptide-binding protein) in Escherichia coli.
Kashiwagi K, Miyaji A, Ikeda S, Tobe T, Sasakawa C, Igarashi K. Kashiwagi K, et al. J Bacteriol. 1992 Jul;174(13):4331-7. doi: 10.1128/jb.174.13.4331-4337.1992. J Bacteriol. 1992. PMID: 1624428 Free PMC article.
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
Weinrauch Y, Msadek T, Kunst F, Dubnau D. Weinrauch Y, et al. J Bacteriol. 1991 Sep;173(18):5685-93. doi: 10.1128/jb.173.18.5685-5693.1991. J Bacteriol. 1991. PMID: 1715859 Free PMC article.
Role of PhoU in phosphate transport and alkaline phosphatase regulation.
Muda M, Rao NN, Torriani A. Muda M, et al. J Bacteriol. 1992 Dec;174(24):8057-64. doi: 10.1128/jb.174.24.8057-8064.1992. J Bacteriol. 1992. PMID: 1459954 Free PMC article.

References

1. Proc Natl Acad Sci U S A. 1964 Mar;51:480-7 - PubMed
1. J Biol Chem. 1977 Jan 10;252(1):268-72 - PubMed
1. Mol Microbiol. 1990 Jun;4(6):873-80 - PubMed
1. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6201-5 - PubMed
1. J Bacteriol. 1987 Jul;169(7):3104-9 - PubMed

The spo0K locus of Bacillus subtilis is homologous to the oligopeptide permease locus and is required for sporulation and competence - PubMed (original) (raw)