Recombinase-mediated gene activation and site-specific integration in mammalian cells - PubMed (original) (raw)
. 1991 Mar 15;251(4999):1351-5.
doi: 10.1126/science.1900642.
Affiliations
- PMID: 1900642
- DOI: 10.1126/science.1900642
Recombinase-mediated gene activation and site-specific integration in mammalian cells
S O'Gorman et al. Science. 1991.
Abstract
A binary system for gene activation and site-specific integration, based on the conditional recombination of transfected sequences mediated by the FLP recombinase from yeast, was implemented in mammalian cells. In several cell lines, FLP rapidly and precisely recombined copies of its specific target sequence to activate an otherwise silent beta-galactosidase reporter gene. Clones of marked cells were generated by excisional recombination within a chromosomally integrated copy of the silent reporter. By the reverse reaction, integration of transfected DNA was targeted to a specific chromosomal site. The results suggest that FLP could be used to mosaically activate or inactivate transgenes for analysis of vertebrate development, and to efficiently integrate transfected DNA at predetermined chromosomal locations.
Similar articles
- Site specific integration of FLP recombinase in BHK-21 cell line.
Ellaiah P, Prakash GJ, Prabhakar T, Saisha V. Ellaiah P, et al. Indian J Exp Biol. 2003 Jun;41(6):570-3. Indian J Exp Biol. 2003. PMID: 15266901 - A modular set of Flp, FRT and lacZ fusion vectors for manipulating genes by site-specific recombination.
Dymecki SM. Dymecki SM. Gene. 1996 Jun 1;171(2):197-201. doi: 10.1016/0378-1119(96)00035-2. Gene. 1996. PMID: 8666272 - FLP-mediated site-specific recombination in microinjected murine zygotes.
Ludwig DL, Stringer JR, Wight DC, Doetschman HC, Duffy JJ. Ludwig DL, et al. Transgenic Res. 1996 Nov;5(6):385-95. doi: 10.1007/BF01980203. Transgenic Res. 1996. PMID: 8840521 - Site-specific gene targeting for gene expression in eukaryotes.
Gorman C, Bullock C. Gorman C, et al. Curr Opin Biotechnol. 2000 Oct;11(5):455-60. doi: 10.1016/s0958-1669(00)00127-0. Curr Opin Biotechnol. 2000. PMID: 11024363 Review. - Site-specific transgene insertion: an approach.
Wigley P, Becker C, Beltrame J, Blake T, Crocker L, Harrison S, Lyons I, McKenzie Z, Tearle R, Crawford R, et al. Wigley P, et al. Reprod Fertil Dev. 1994;6(5):585-8. doi: 10.1071/rd9940585. Reprod Fertil Dev. 1994. PMID: 7569037 Review.
Cited by
- Microbiome and Human Health: Current Understanding, Engineering, and Enabling Technologies.
Aggarwal N, Kitano S, Puah GRY, Kittelmann S, Hwang IY, Chang MW. Aggarwal N, et al. Chem Rev. 2023 Jan 11;123(1):31-72. doi: 10.1021/acs.chemrev.2c00431. Epub 2022 Nov 1. Chem Rev. 2023. PMID: 36317983 Free PMC article. Review. - A nanobody-based strategy for rapid and scalable purification of human protein complexes.
Stevens TA, Tomaleri GP, Hazu M, Wei S, Nguyen VN, DeKalb C, Voorhees RM, Pleiner T. Stevens TA, et al. Nat Protoc. 2024 Jan;19(1):127-158. doi: 10.1038/s41596-023-00904-w. Epub 2023 Nov 16. Nat Protoc. 2024. PMID: 37974029 Review. - The herpes simplex virus 2 UL21 protein is essential for virus propagation.
Le Sage V, Jung M, Alter JD, Wills EG, Johnston SM, Kawaguchi Y, Baines JD, Banfield BW. Le Sage V, et al. J Virol. 2013 May;87(10):5904-15. doi: 10.1128/JVI.03489-12. Epub 2013 Mar 13. J Virol. 2013. PMID: 23487471 Free PMC article. - Conditional gene targeting in macrophages and granulocytes using LysMcre mice.
Clausen BE, Burkhardt C, Reith W, Renkawitz R, Förster I. Clausen BE, et al. Transgenic Res. 1999 Aug;8(4):265-77. doi: 10.1023/a:1008942828960. Transgenic Res. 1999. PMID: 10621974 - CNS myelin paranodes require Nkx6-2 homeoprotein transcriptional activity for normal structure.
Southwood C, He C, Garbern J, Kamholz J, Arroyo E, Gow A. Southwood C, et al. J Neurosci. 2004 Dec 15;24(50):11215-25. doi: 10.1523/JNEUROSCI.3479-04.2004. J Neurosci. 2004. PMID: 15601927 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials