Expression of different Jun and Fos proteins during the G0-to-G1 transition in mouse fibroblasts: in vitro and in vivo associations - PubMed (original) (raw)

Expression of different Jun and Fos proteins during the G0-to-G1 transition in mouse fibroblasts: in vitro and in vivo associations

K Kovary et al. Mol Cell Biol. 1991 May.

Abstract

We have characterized the expression of c-Jun, JunB, JunD, c-Fos, and FosB proteins following serum stimulation of quiescent Swiss 3T3 cells by immunoprecipitation analyses. The synthesis of the three Jun proteins rapidly increases following stimulation, remaining at a significant level for at least 8 h. JunB protein presents the highest expression of all. FosB, like c-Fos, is transiently induced. Pulse-chase experiments show that all of the proteins except JunD are short-lived. We have shown that c-Fos and FosB form complexes in vivo with the different Jun proteins and that JunB complexes are predominant. In vitro association and competition experiments show that the affinities between the different Fos and Jun proteins are similar. This finding, together with the in vivo observations described above, suggests that the proportion of the different Jun/Fos heterodimers is governed by the concentration of the different components. The Fos and Jun proteins are phosphoproteins, and some remain relatively highly phosphorylated in their heterodimeric form.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1487-91 - PubMed
    1. Nature. 1988 Dec 15;336(6200):646-51 - PubMed
    1. Mol Cell Biol. 1988 May;8(5):2063-9 - PubMed
    1. Science. 1987 Dec 4;238(4832):1386-92 - PubMed
    1. Science. 1988 May 20;240(4855):1010-6 - PubMed

MeSH terms

Substances

LinkOut - more resources