An A + U-rich element RNA-binding factor regulates c-myc mRNA stability in vitro - PubMed (original) (raw)

An A + U-rich element RNA-binding factor regulates c-myc mRNA stability in vitro

G Brewer. Mol Cell Biol. 1991 May.

Abstract

Transient expression of some proto-oncogenes, cytokines, and transcription factors occurs as a cellular response to growth factors, 12-O-tetradecanoylphorbol-13-acetate, antigen stimulation, or inflammation. Expression of these genes is mediated in part by the rapid turnover of their mRNAs. A + U-rich elements in the 3' untranslated regions of these mRNAs serve as one recognition signal targeting the mRNAs for rapid degradation. I report the identification of a cytosolic factor that both binds to the proto-oncogene c-myc A + U-rich element and specifically destabilizes c-myc mRNA in a cell-free mRNA decay system which reconstitutes mRNA decay processes found in cells. Proteinase K treatment of the factor abolishes its c-myc mRNA degradation activity without affecting its RNA-binding capacity. Thus, RNA substrate binding and degradation appear to be separable functions. These findings should aid in understanding how the cell selectively targets mRNAs for rapid turnover.

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References

1. Mol Cell Biol. 1988 Aug;8(8):3244-50 - PubMed
1. Nature. 1985 Oct 3-9;317(6036):443-5 - PubMed
1. Oncogene Res. 1988 Sep;3(2):155-66 - PubMed
1. Cell. 1986 Aug 29;46(5):659-67 - PubMed
1. J Mol Biol. 1986 Apr 20;188(4):579-93 - PubMed

An A + U-rich element RNA-binding factor regulates c-myc mRNA stability in vitro - PubMed (original) (raw)