Inhibition of localized adhesion of enteropathogenic Escherichia coli to HEp-2 cells by immunoglobulin and oligosaccharide fractions of human colostrum and breast milk - PubMed (original) (raw)

Inhibition of localized adhesion of enteropathogenic Escherichia coli to HEp-2 cells by immunoglobulin and oligosaccharide fractions of human colostrum and breast milk

A Cravioto et al. J Infect Dis. 1991 Jun.

Abstract

Secretory IgA (sIgA) purified from colostrum and breast milk obtained from 14 women inhibited the localized adherence of an enteropathogenic Escherichia coli (EPEC) to HEp-2 cells. Inhibition decreased as lactation continued even when the concentration of sIgA was maintained constant at 1 mg/ml. sIgA responded to a 94-kDa plasmid-encoded outer membrane protein implicated as the EPEC adherence factor. An oligosaccharide-enriched fraction (OEF) from these samples also inhibited the attachment of this EPEC. Inhibition by OEFs decreased as lactation continued because of a general reduction in oligosaccharide content. Localized adherence of six other EPEC was also inhibited by sIgA and OEF, whereas attachment of isolates with diffuse or aggregative adherence was not inhibited by these fractions. Experiments with purified oligosaccharide fractions revealed that EPEC attach to HEp-2 cells through a carbohydrate-mediated mechanism based on the preferential recognition of fucosylated residues in human milk.

PubMed Disclaimer

Similar articles

• Effect of microwave radiation, pasteurization and lyophilization on the ability of human milk to inhibit Escherichia coli adherence to HEp-2 cells.
  Carbonare SB, Palmeira P, Silva ML, Carneiro-Sampaio MM. Carbonare SB, et al. J Diarrhoeal Dis Res. 1996 Jun;14(2):90-4. J Diarrhoeal Dis Res. 1996. PMID: 8870401
• Inhibition of enteropathogenic Escherichia coli (EPEC) adhesion to HeLa cells by human colostrum: detection of specific sIgA related to EPEC outer-membrane proteins.
  Camara LM, Carbonare SB, Silva ML, Carneiro-Sampaio MM. Camara LM, et al. Int Arch Allergy Immunol. 1994;103(3):307-10. doi: 10.1159/000236645. Int Arch Allergy Immunol. 1994. PMID: 8111250
• Inhibition of enteropathogenic Escherichia coli (EPEC) adherence to HeLa cells by human colostrum. Detection of specific sIgA related to EPEC outer-membrane proteins.
  Câmara LM, Carbonare SB, Scaletsky IC, da Silva ML, Carneiro-Sampaio MM. Câmara LM, et al. Adv Exp Med Biol. 1995;371A:673-6. doi: 10.1007/978-1-4615-1941-6_142. Adv Exp Med Biol. 1995. PMID: 8526016 No abstract available.
• The immune response of the mammary gland and its significance for the neonate.
  Hanson LA, Ahlstedt S, Andersson B, Cruz JR, Dahlgren U, Fällström SP, Porras O, Svanborg Edén C, Söderström T, Wettergren B. Hanson LA, et al. Ann Allergy. 1984 Dec;53(6 Pt 2):576-82. Ann Allergy. 1984. PMID: 6391286 Review.

Cited by

• Shaping infant development from the inside out: Bioactive factors in human milk.
  Andres SF, Scottoline B, Good M. Andres SF, et al. Semin Perinatol. 2023 Feb;47(1):151690. doi: 10.1016/j.semperi.2022.151690. Epub 2022 Dec 21. Semin Perinatol. 2023. PMID: 36581527 Free PMC article. Review.
• Adhesion and its role in the virulence of enteropathogenic Escherichia coli.
  Law D. Law D. Clin Microbiol Rev. 1994 Apr;7(2):152-73. doi: 10.1128/CMR.7.2.152. Clin Microbiol Rev. 1994. PMID: 8055465 Free PMC article. Review.
• The Human Milk Glycome as a Defense Against Infectious Diseases: Rationale, Challenges, and Opportunities.
  Craft KM, Townsend SD. Craft KM, et al. ACS Infect Dis. 2018 Feb 9;4(2):77-83. doi: 10.1021/acsinfecdis.7b00209. Epub 2017 Nov 15. ACS Infect Dis. 2018. PMID: 29140081 Free PMC article. Review.
• Consumption of human milk oligosaccharides by gut-related microbes.
  Marcobal A, Barboza M, Froehlich JW, Block DE, German JB, Lebrilla CB, Mills DA. Marcobal A, et al. J Agric Food Chem. 2010 May 12;58(9):5334-40. doi: 10.1021/jf9044205. J Agric Food Chem. 2010. PMID: 20394371 Free PMC article.
• Decreased adherence of enterohemorrhagic Escherichia coli to HEp-2 cells in the presence of antibodies that recognize the C-terminal region of intimin.
  Gansheroff LJ, Wachtel MR, O'Brien AD. Gansheroff LJ, et al. Infect Immun. 1999 Dec;67(12):6409-17. doi: 10.1128/IAI.67.12.6409-6417.1999. Infect Immun. 1999. PMID: 10569757 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database

• Miscellaneous

  • NCI CPTAC Assay Portal