Concentration of GPI-anchored proteins upon ER exit in yeast - PubMed (original) (raw)

. 2009 Feb;10(2):186-200.

doi: 10.1111/j.1600-0854.2008.00857.x. Epub 2008 Nov 20.

Affiliations

• PMID: 19054390
• DOI: 10.1111/j.1600-0854.2008.00857.x

Free article

Concentration of GPI-anchored proteins upon ER exit in yeast

Guillaume A Castillon et al. Traffic. 2009 Feb.

Free article

Abstract

Previous biochemical work has revealed two parallel routes of exit from the endoplasmic reticulum (ER) in the yeast Saccharomyces cerevisiae, one seemingly specific for glycosyl-phosphatidylinositol (GPI)-anchored proteins. Using the coat protein II (COPII) mutant sec31-1, we visualized ER exit sites (ERES) and identified three distinct ERES populations in vivo. One contains glycosylated pro-alpha-factor, the second contains the GPI-anchored proteins Cwp2p, Ccw14p and Tos6p and the third is enriched with the hexose transporter, Hxt1p. Concentration of GPI-anchored proteins prior to budding requires anchor remodeling, and Hxt1p incorporation into ERES requires the COPII components Sec12p and Sec16p. Additionally, we have found that GPI-anchored protein ER exit is controlled by the p24 family member Emp24p, whereas ER export of most transmembrane proteins requires the Cornichon homologue Erv14p.

PubMed Disclaimer

Similar articles

• The presence of an ER exit signal determines the protein sorting upon ER exit in yeast.
  Watanabe R, Castillon GA, Meury A, Riezman H. Watanabe R, et al. Biochem J. 2008 Sep 1;414(2):237-45. doi: 10.1042/BJ20080715. Biochem J. 2008. PMID: 18462190
• The ER v-SNAREs are required for GPI-anchored protein sorting from other secretory proteins upon exit from the ER.
  Morsomme P, Prescianotto-Baschong C, Riezman H. Morsomme P, et al. J Cell Biol. 2003 Aug 4;162(3):403-12. doi: 10.1083/jcb.200212101. Epub 2003 Jul 28. J Cell Biol. 2003. PMID: 12885760 Free PMC article.
• Selective export of human GPI-anchored proteins from the endoplasmic reticulum.
  Bonnon C, Wendeler MW, Paccaud JP, Hauri HP. Bonnon C, et al. J Cell Sci. 2010 May 15;123(Pt 10):1705-15. doi: 10.1242/jcs.062950. Epub 2010 Apr 27. J Cell Sci. 2010. PMID: 20427317
• Differential ER exit in yeast and mammalian cells.
  Watanabe R, Riezman H. Watanabe R, et al. Curr Opin Cell Biol. 2004 Aug;16(4):350-5. doi: 10.1016/j.ceb.2004.06.010. Curr Opin Cell Biol. 2004. PMID: 15261666 Review.
• Transport of glycosylphosphatidylinositol-anchored proteins from the endoplasmic reticulum.
  Kinoshita T, Maeda Y, Fujita M. Kinoshita T, et al. Biochim Biophys Acta. 2013 Nov;1833(11):2473-8. doi: 10.1016/j.bbamcr.2013.01.027. Epub 2013 Feb 1. Biochim Biophys Acta. 2013. PMID: 23380706 Review.

Cited by

• Vesicle-mediated export from the ER: COPII coat function and regulation.
  D'Arcangelo JG, Stahmer KR, Miller EA. D'Arcangelo JG, et al. Biochim Biophys Acta. 2013 Nov;1833(11):2464-72. doi: 10.1016/j.bbamcr.2013.02.003. Epub 2013 Feb 15. Biochim Biophys Acta. 2013. PMID: 23419775 Free PMC article. Review.
• Traffic of p24 Proteins and COPII Coat Composition Mutually Influence Membrane Scaffolding.
  D'Arcangelo JG, Crissman J, Pagant S, Čopič A, Latham CF, Snapp EL, Miller EA. D'Arcangelo JG, et al. Curr Biol. 2015 May 18;25(10):1296-305. doi: 10.1016/j.cub.2015.03.029. Epub 2015 Apr 30. Curr Biol. 2015. PMID: 25936552 Free PMC article.
• Insights into structural and regulatory roles of Sec16 in COPII vesicle formation at ER exit sites.
  Yorimitsu T, Sato K. Yorimitsu T, et al. Mol Biol Cell. 2012 Aug;23(15):2930-42. doi: 10.1091/mbc.E12-05-0356. Epub 2012 Jun 6. Mol Biol Cell. 2012. PMID: 22675024 Free PMC article.
• SEC14 is a specific requirement for secretion of phospholipase B1 and pathogenicity of Cryptococcus neoformans.
  Chayakulkeeree M, Johnston SA, Oei JB, Lev S, Williamson PR, Wilson CF, Zuo X, Leal AL, Vainstein MH, Meyer W, Sorrell TC, May RC, Djordjevic JT. Chayakulkeeree M, et al. Mol Microbiol. 2011 May;80(4):1088-101. doi: 10.1111/j.1365-2958.2011.07632.x. Epub 2011 Mar 31. Mol Microbiol. 2011. PMID: 21453402 Free PMC article.
• Involvement of the penta-EF-hand protein Pef1p in the Ca2+-dependent regulation of COPII subunit assembly in Saccharomyces cerevisiae.
  Yoshibori M, Yorimitsu T, Sato K. Yoshibori M, et al. PLoS One. 2012;7(7):e40765. doi: 10.1371/journal.pone.0040765. Epub 2012 Jul 11. PLoS One. 2012. PMID: 22792405 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Molecular Biology Databases

  • BioCyc
  • Saccharomyces Genome Database