Thrombin decreases glycosaminoglycans content of endothelial cells in culture - PubMed (original) (raw)
Thrombin decreases glycosaminoglycans content of endothelial cells in culture
T Kaji et al. Thromb Res. 1991.
Abstract
The effect of thrombin on the metabolism of glycosaminoglycans (GAG) in cultured bovine aortic endothelial cells was investigated. Incubation of confluent endothelial cell cultures with thrombin at 1.0 NIH U/ml for 8 h and above caused a significant decrease in the accumulation of [35S]sulfate-labeled GAG (35S-GAG) in the cell layer. After a 24 h incubation, thrombin at 0.5 U/ml and above decreased the accumulation of 35S-GAG in both cell layer and medium. The percentage of 35S-GAG released into the medium during the last 3 h of 24 h incubation was significantly increased by thrombin. In thrombin-treated cell layer, both heparan sulfate and the other GAG was significantly decreased at the same degree. The incorporation of both [3H]thymidine and [14C]leucine was significantly increased by thrombin. Gabexate mesilate, a serine protease inhibitor, suppressed the thrombin inhibition. From these results, it was suggested that thrombin decreased 35S-GAG in the endothelial cell layer without a nonspecific damage of the cells through mainly an inhibition of GAG production and additionally a stimulation of GAG release.
Similar articles
- Effect of lead on the glycosaminoglycans metabolism of bovine aortic endothelial cells in culture.
Kaji T, Yamamoto C, Sakamoto M. Kaji T, et al. Toxicology. 1991;68(3):249-57. doi: 10.1016/0300-483x(91)90073-a. Toxicology. 1991. PMID: 1896998 - Antithrombin III modulates the effect of thrombin on the metabolism of glycosaminoglycans in cultured endothelial cells.
Akai T, Kaji T, Hayakawa Y, Hayashi T, Sakuragawa N. Akai T, et al. Thromb Res. 1991 Jun 15;62(6):707-16. doi: 10.1016/0049-3848(91)90374-6. Thromb Res. 1991. PMID: 1926061 - Effect of thrombin on the production of glycosaminoglycans by cultured endothelial cells.
Kaji T, Akai T, Hayashi T, Sakuragawa N. Kaji T, et al. Thromb Res. 1991 Jun 1;62(5):509-17. doi: 10.1016/0049-3848(91)90024-q. Thromb Res. 1991. PMID: 1896966 - Stimulation of heparan sulphate release from cultured endothelial cells by plasmin.
Kaji T, Sakamoto M. Kaji T, et al. Blood Coagul Fibrinolysis. 1991 Jun;2(3):419-23. doi: 10.1097/00001721-199106000-00003. Blood Coagul Fibrinolysis. 1991. PMID: 1834236 - Phorbol 12-myristate 13-acetate stimulates the release of glycosaminoglycans from cultured vascular endothelial cells: possible involvement of protein kinase C activation.
Fujii N, Kaji T, Yamamoto C, Fujiwara Y, Koizumi F. Fujii N, et al. Thromb Res. 1996 Jun 1;82(5):379-87. doi: 10.1016/0049-3848(96)00087-4. Thromb Res. 1996. PMID: 8771698
Cited by
- Identification of the predominant glycosaminoglycan-attachment site in soluble recombinant human thrombomodulin: potential regulation of functionality by glycosyltransferase competition for serine474.
Gerlitz B, Hassell T, Vlahos CJ, Parkinson JF, Bang NU, Grinnell BW. Gerlitz B, et al. Biochem J. 1993 Oct 1;295 ( Pt 1)(Pt 1):131-40. doi: 10.1042/bj2950131. Biochem J. 1993. PMID: 8216207 Free PMC article.