Characterization of DNA-binding proteins using multiplexed competitor EMSA - PubMed (original) (raw)
. 2009 Jan 23;385(3):714-7.
doi: 10.1016/j.jmb.2008.11.035. Epub 2008 Nov 27.
Affiliations
- PMID: 19059416
- DOI: 10.1016/j.jmb.2008.11.035
Characterization of DNA-binding proteins using multiplexed competitor EMSA
Andrew J P Smith et al. J Mol Biol. 2009.
Abstract
We describe a low-cost high-throughput technique to characterize nuclear protein DNA-binding interactions. This technique, known as Multiplexed Competitor Electrophoretic Mobility Shift Assay, uses a series of multiplexed oligonucleotide DNA consensus competitors, in combination with a standard electrophoretic mobility shift assay procedure, to efficiently characterize DNA-binding proteins. We show utility for the method to identify a previously unreported hepatocyte nuclear factor-3 site created in intron 8 of the lipoprotein lipase gene by a common single-nucleotide polymorphism (rs327).
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