Inhibition of measles virus infections in cell cultures by peptide-conjugated morpholino oligomers - PubMed (original) (raw)
Inhibition of measles virus infections in cell cultures by peptide-conjugated morpholino oligomers
Katrina Sleeman et al. Virus Res. 2009 Mar.
Abstract
Measles virus (MeV) is a highly contagious human pathogen. Despite the success of measles vaccination programs, measles is still responsible for an estimated 245,000 deaths each year. There are currently no antiviral compounds available for the treatment of measles. Peptide-conjugated phosphorodiamidate morpholino oligomers (PPMO) are antisense compounds that enter cells readily and can interfere with mRNA function by steric blocking. A panel of PPMO was designed to target various sequences of MeV RNA that are known to be important for viral replication. Five PPMO, targeting MeV genomic RNA or mRNA, inhibited the replication of MeV, in a dose-responsive and sequence-specific manner in cultured cells. One of the highly active PPMO (PPMO 454), targeting a conserved sequence in the translation start site of the mRNA coding for the nucleocapsid protein, inhibited multiple genotypes of MeV. This report provides evidence that PPMO treatment represents a promising approach for developing antiviral agents against measles and other paramyxoviruses.
Similar articles
- Inhibition of RNA virus infections with peptide-conjugated morpholino oligomers.
Stein DA. Stein DA. Curr Pharm Des. 2008;14(25):2619-34. doi: 10.2174/138161208786071290. Curr Pharm Des. 2008. PMID: 18991679 Review. - In vitro resistance selection and in vivo efficacy of morpholino oligomers against West Nile virus.
Deas TS, Bennett CJ, Jones SA, Tilgner M, Ren P, Behr MJ, Stein DA, Iversen PL, Kramer LD, Bernard KA, Shi PY. Deas TS, et al. Antimicrob Agents Chemother. 2007 Jul;51(7):2470-82. doi: 10.1128/AAC.00069-07. Epub 2007 May 7. Antimicrob Agents Chemother. 2007. PMID: 17485503 Free PMC article. - Inhibition of coxsackievirus B3 in cell cultures and in mice by peptide-conjugated morpholino oligomers targeting the internal ribosome entry site.
Yuan J, Stein DA, Lim T, Qiu D, Coughlin S, Liu Z, Wang Y, Blouch R, Moulton HM, Iversen PL, Yang D. Yuan J, et al. J Virol. 2006 Dec;80(23):11510-9. doi: 10.1128/JVI.00900-06. Epub 2006 Sep 20. J Virol. 2006. PMID: 16987987 Free PMC article. - Inhibition of dengue virus serotypes 1 to 4 in vero cell cultures with morpholino oligomers.
Kinney RM, Huang CY, Rose BC, Kroeker AD, Dreher TW, Iversen PL, Stein DA. Kinney RM, et al. J Virol. 2005 Apr;79(8):5116-28. doi: 10.1128/JVI.79.8.5116-5128.2005. J Virol. 2005. PMID: 15795296 Free PMC article. - Cell-penetrating peptide-morpholino conjugates alter pre-mRNA splicing of DMD (Duchenne muscular dystrophy) and inhibit murine coronavirus replication in vivo.
Moulton HM, Fletcher S, Neuman BW, McClorey G, Stein DA, Abes S, Wilton SD, Buchmeier MJ, Lebleu B, Iversen PL. Moulton HM, et al. Biochem Soc Trans. 2007 Aug;35(Pt 4):826-8. doi: 10.1042/BST0350826. Biochem Soc Trans. 2007. PMID: 17635157 Review.
Cited by
- Antiviral Peptide-Based Conjugates: State of the Art and Future Perspectives.
Todorovski T, Kalafatovic D, Andreu D. Todorovski T, et al. Pharmaceutics. 2023 Jan 20;15(2):357. doi: 10.3390/pharmaceutics15020357. Pharmaceutics. 2023. PMID: 36839679 Free PMC article. Review. - Application of Cell Penetrating Peptides as a Promising Drug Carrier to Combat Viral Infections.
Khairkhah N, Namvar A, Bolhassani A. Khairkhah N, et al. Mol Biotechnol. 2023 Sep;65(9):1387-1402. doi: 10.1007/s12033-023-00679-1. Epub 2023 Jan 31. Mol Biotechnol. 2023. PMID: 36719639 Free PMC article. Review. - Evolution of complexity in non-viral oligonucleotide delivery systems: from gymnotic delivery through bioconjugates to biomimetic nanoparticles.
Bakowski K, Vogel S. Bakowski K, et al. RNA Biol. 2022 Jan;19(1):1256-1275. doi: 10.1080/15476286.2022.2147278. RNA Biol. 2022. PMID: 36411594 Free PMC article. Review. - Repurposing an In Vitro Measles Virus Dissemination Assay for Screening of Antiviral Compounds.
Schmitz KS, Lange MV, Gommers L, Handrejk K, Porter DP, Alabi CA, Moscona A, Porotto M, de Vries RD, de Swart RL. Schmitz KS, et al. Viruses. 2022 May 29;14(6):1186. doi: 10.3390/v14061186. Viruses. 2022. PMID: 35746658 Free PMC article. Review. - Antisense Phosphorodiamidate Morpholino Oligomers as Novel Antiviral Compounds.
Nan Y, Zhang YJ. Nan Y, et al. Front Microbiol. 2018 Apr 20;9:750. doi: 10.3389/fmicb.2018.00750. eCollection 2018. Front Microbiol. 2018. PMID: 29731743 Free PMC article. Review.