Kinetic analysis of amyloid fibril polymerization in vitro - PubMed (original) (raw)
Affiliations
- PMID: 1906561
Kinetic analysis of amyloid fibril polymerization in vitro
H Naiki et al. Lab Invest. 1991 Jul.
Erratum in
- Lab Invest 1992 Nov;67(5):652
Abstract
We investigated the polymerization kinetics of murine senile amyloid fibrils (fASSAM) in vitro. When sonicated murine senile amyloid fibrils was incubated with its constituent monomer protein, the extension of amyloid fibrils was observed in an electron microscopic analysis. Quantitative fluorometric analysis with thioflavine T (Naiki H, Higuchi K, Hosokawa M, Takeda T: Anal Biochem 177:244, 1989) revealed that (a) extension of amyloid fibrils occurred by a pseudo-first-order exponential increase in the fluorescence of thioflavine T; (b) the rate of extension was maximal around pH 7.5, and was inhibited with the increase in KCl or NaCl concentration in the reaction mixture; (c) the rate of polymerization was proportional to the product of the murine senile amyloid fibrils number concentration and the constituent monomer protein concentration; (d) the net rate of extension was the sum of the rates of polymerization and depolymerization with the equilibrium association constant K of 5 x 10(7) M-1. These results show that amyloid fibril formation can apparently be explained by a first-order kinetic model: that is, extension of amyloid fibrils proceeds by consecutive association of precursor proteins onto the ends of existing fibrils.
Similar articles
- First-order kinetic model of Alzheimer's beta-amyloid fibril extension in vitro.
Naiki H, Nakakuki K. Naiki H, et al. Lab Invest. 1996 Feb;74(2):374-83. Lab Invest. 1996. PMID: 8780157 - Fluorometric examination of tissue amyloid fibrils in murine senile amyloidosis: use of the fluorescent indicator, thioflavine T.
Naiki H, Higuchi K, Matsushima K, Shimada A, Chen WH, Hosokawa M, Takeda T. Naiki H, et al. Lab Invest. 1990 Jun;62(6):768-73. Lab Invest. 1990. PMID: 2359260 - Concentration-dependent inhibitory effects of apolipoprotein E on Alzheimer's beta-amyloid fibril formation in vitro.
Naiki H, Gejyo F, Nakakuki K. Naiki H, et al. Biochemistry. 1997 May 20;36(20):6243-50. doi: 10.1021/bi9624705. Biochemistry. 1997. PMID: 9166797 - Amyloid fibril formation and disaggregation of fragment 1-29 of apomyoglobin: insights into the effect of pH on protein fibrillogenesis.
Picotti P, De Franceschi G, Frare E, Spolaore B, Zambonin M, Chiti F, de Laureto PP, Fontana A. Picotti P, et al. J Mol Biol. 2007 Apr 13;367(5):1237-45. doi: 10.1016/j.jmb.2007.01.072. Epub 2007 Feb 3. J Mol Biol. 2007. PMID: 17320902 Review. - Molecular interactions in the formation and deposition of beta2-microglobulin-related amyloid fibrils.
Naiki H, Yamamoto S, Hasegawa K, Yamaguchi I, Goto Y, Gejyo F. Naiki H, et al. Amyloid. 2005 Mar;12(1):15-25. doi: 10.1080/13506120500032352. Amyloid. 2005. PMID: 16076607 Review.
Cited by
- Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils-current status.
Groenning M. Groenning M. J Chem Biol. 2010 Mar;3(1):1-18. doi: 10.1007/s12154-009-0027-5. Epub 2009 Aug 20. J Chem Biol. 2010. PMID: 19693614 Free PMC article. - A novel assay in vitro of human islet amyloid polypeptide amyloidogenesis and effects of insulin secretory vesicle peptides on amyloid formation.
Kudva YC, Mueske C, Butler PC, Eberhardt NL. Kudva YC, et al. Biochem J. 1998 May 1;331 ( Pt 3)(Pt 3):809-13. doi: 10.1042/bj3310809. Biochem J. 1998. PMID: 9560308 Free PMC article. - Amyloid formation of growth hormone in presence of zinc: Relevance to its storage in secretory granules.
Jacob RS, Das S, Ghosh S, Anoop A, Jha NN, Khan T, Singru P, Kumar A, Maji SK. Jacob RS, et al. Sci Rep. 2016 Mar 23;6:23370. doi: 10.1038/srep23370. Sci Rep. 2016. PMID: 27004850 Free PMC article. - Amyloid-DNA Composites of Bacterial Biofilms Stimulate Autoimmunity.
Gallo PM, Rapsinski GJ, Wilson RP, Oppong GO, Sriram U, Goulian M, Buttaro B, Caricchio R, Gallucci S, Tükel Ç. Gallo PM, et al. Immunity. 2015 Jun 16;42(6):1171-84. doi: 10.1016/j.immuni.2015.06.002. Immunity. 2015. PMID: 26084027 Free PMC article. - REVIEW: Curcumin and Alzheimer's disease.
Hamaguchi T, Ono K, Yamada M. Hamaguchi T, et al. CNS Neurosci Ther. 2010 Oct;16(5):285-97. doi: 10.1111/j.1755-5949.2010.00147.x. CNS Neurosci Ther. 2010. PMID: 20406252 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources