An atypical RNA polymerase involved in RNA silencing shares small subunits with RNA polymerase II - PubMed (original) (raw)

doi: 10.1038/nsmb.1539. Epub 2008 Dec 14.

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• PMID: 19079263
• DOI: 10.1038/nsmb.1539

An atypical RNA polymerase involved in RNA silencing shares small subunits with RNA polymerase II

Linfeng Huang et al. Nat Struct Mol Biol. 2009 Jan.

Abstract

Genetic evidence indicates that plant-specific homologs of DNA-dependent RNA polymerase (Pol) II large subunits form Pol IV and Pol V complexes involved in small interfering RNA production and RNA-directed DNA methylation. Here we describe evidence that Pol V contains subunits shared with Pol II, but that RNA polymerase II subunit (RPB)-4 is missing from Pol V and that RPB5 is present as a Pol V-specific isomer, RPB5b. Pol V also has other proteins that are not present in Pol II, consistent with a role of this complex as an effector of silencing.

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