Electrotransformation in Salmonella typhimurium LT2 - PubMed (original) (raw)
Electrotransformation in Salmonella typhimurium LT2
J Binotto et al. Can J Microbiol. 1991 Jun.
Abstract
Electroporation gives high efficiency of transformation in Salmonella typhimurium LT2, yielding 10(8)-10(9) electrotransformants per microgram of pBR322 DNA. Lipopolysaccharide (LPS) composition has little influence on electrotransformation efficiency by electroporation, unlike Ca2+ shock methods, which give ca. 10(6) transformants/microgram DNA with strains with Rc or Rd2 LPS, 10(4) transformants with most smooth and rough strains, and 10(2) transformants with strains with Re LPS. Thus cell envelope properties are less crucial in electrotransformation than in Ca2+ shock methods. The reciprocal restriction barrier between Escherichia coli K-12 and S. typhimurium LT2 reduces electrotransformation by ca. 100-fold, but host-restriction mutants reduce or eliminate the barrier.
Similar articles
- High efficiency transformation of Salmonella typhimurium and Salmonella typhi by electroporation.
O'Callaghan D, Charbit A. O'Callaghan D, et al. Mol Gen Genet. 1990 Aug;223(1):156-8. doi: 10.1007/BF00315809. Mol Gen Genet. 1990. PMID: 2259337 - Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains.
MacLachlan PR, Sanderson KE. MacLachlan PR, et al. J Bacteriol. 1985 Jan;161(1):442-5. doi: 10.1128/jb.161.1.442-445.1985. J Bacteriol. 1985. PMID: 3881397 Free PMC article. - The influence of the growth phase of enteric bacteria on electrotransformation with plasmid DNA.
Szostková M, Horáková D, Nĕmec M. Szostková M, et al. Folia Microbiol (Praha). 1999;44(2):177-80. doi: 10.1007/BF02816238. Folia Microbiol (Praha). 1999. PMID: 10588053 - Optimization of electrotransformation (ETF) conditions in lactic acid bacteria (LAB).
Wang C, Cui Y, Qu X. Wang C, et al. J Microbiol Methods. 2020 Jul;174:105944. doi: 10.1016/j.mimet.2020.105944. Epub 2020 May 15. J Microbiol Methods. 2020. PMID: 32417130 Review. - Bacterial genetics by electric shock.
Solioz M, Bienz D. Solioz M, et al. Trends Biochem Sci. 1990 May;15(5):175-7. doi: 10.1016/0968-0004(90)90154-4. Trends Biochem Sci. 1990. PMID: 2193434 Review.
Cited by
- Functional analysis of the galactosyltransferases required for biosynthesis of D-galactan I, a component of the lipopolysaccharide O1 antigen of Klebsiella pneumoniae.
Guan S, Clarke AJ, Whitfield C. Guan S, et al. J Bacteriol. 2001 Jun;183(11):3318-27. doi: 10.1128/JB.183.11.3318-3327.2001. J Bacteriol. 2001. PMID: 11344139 Free PMC article. - Structure of the core oligosaccharide in the serotype O8 lipopolysaccharide from Klebsiella pneumoniae.
Severn WB, Kelly RF, Richards JC, Whitfield C. Severn WB, et al. J Bacteriol. 1996 Mar;178(6):1731-41. doi: 10.1128/jb.178.6.1731-1741.1996. J Bacteriol. 1996. PMID: 8626303 Free PMC article. - The multi-drug efflux system AcrABZ-TolC is essential for infection of Salmonella Typhimurium by the flagellum-dependent bacteriophage Chi.
Esteves NC, Porwollik S, McClelland M, Scharf BE. Esteves NC, et al. J Virol. 2021 May 10;95(11):e00394-21. doi: 10.1128/JVI.00394-21. Epub 2021 Mar 17. J Virol. 2021. PMID: 33731456 Free PMC article. - Identification and functional characterization of an ABC transport system involved in polysaccharide export of A-band lipopolysaccharide in Pseudomonas aeruginosa.
Rocchetta HL, Lam JS. Rocchetta HL, et al. J Bacteriol. 1997 Aug;179(15):4713-24. doi: 10.1128/jb.179.15.4713-4724.1997. J Bacteriol. 1997. PMID: 9244257 Free PMC article. - Systemic and mucosal immune responses in mice orally immunized with avirulent Salmonella typhimurium expressing a cloned Porphyromonas gingivalis hemagglutinin.
Dusek DM, Progulske-Fox A, Brown TA. Dusek DM, et al. Infect Immun. 1994 May;62(5):1652-7. doi: 10.1128/iai.62.5.1652-1657.1994. Infect Immun. 1994. PMID: 8168925 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Miscellaneous