Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis - PubMed (original) (raw)
Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis
R C Parker et al. Proc Natl Acad Sci U S A. 1977 Mar.
Abstract
The cleavage of DNA by restriction endonucleases can be limited by the addition of ethidium bromide. When closed circular DNA is used as a substrate, DNA with one-site cleavages of one or both strands can be made by adding appropriate amounts of dye. The singly cleaved DNA is a complete set of full-length permuted linear molecules. Fractionation of the products of a digestion of the permuted linears with a single-hitting restriction endonuclease by gel electrophoresis yields a series of bands that can be used to determine relative molecular weights of the DNA fragments in the gel without the introduction of standards. It is possible to determine the relative molecular weight of a fragment to within +/-2.5%. These molecular weights immediately allow the determination of the HindIII and Hpa I maps of simian virus 40. The HindIII map of bacteriophage PM2 was determined by this method with one ambiguity that was resolved by using traditional techniques.
Similar articles
- Restriction endonuclease cleavage of linear and closed circular murine leukemia viral DNAs: discovery of a smaller circular form.
Yoshimura FK, Weinberg RA. Yoshimura FK, et al. Cell. 1979 Feb;16(2):323-32. doi: 10.1016/0092-8674(79)90009-6. Cell. 1979. PMID: 455438 - Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.
Helling RB, Goodman HM, Boyer HW. Helling RB, et al. J Virol. 1974 Nov;14(5):1235-44. doi: 10.1128/JVI.14.5.1235-1244.1974. J Virol. 1974. PMID: 4372397 Free PMC article. - Ethidium-bromide-inhibited restriction endonucleases cleave one strand of circular DNA.
Osterlund M, Luthman H, Nilsson SV, Magnusson G. Osterlund M, et al. Gene. 1982 Nov;20(1):121-5. doi: 10.1016/0378-1119(82)90093-2. Gene. 1982. PMID: 6298065 - Mapping of cleavage sites for restriction endonucleases in lambdadv plasmids.
Streeck RE, Hobom G. Streeck RE, et al. Eur J Biochem. 1975 Sep 15;57(2):595-606. doi: 10.1111/j.1432-1033.1975.tb02335.x. Eur J Biochem. 1975. PMID: 809288 - Pulsed field agarose gel electrophoresis in the study of morphogenesis: packaging of double-stranded DNA in the capsids of bacteriophages.
Serwer P, Hayes SJ, Moreno ET, Louie D, Watson RH, Son M. Serwer P, et al. Electrophoresis. 1993 Apr;14(4):271-7. doi: 10.1002/elps.1150140148. Electrophoresis. 1993. PMID: 8500457 Review.
Cited by
- Rapid evolution of animal mitochondrial DNA.
Brown WM, George M Jr, Wilson AC. Brown WM, et al. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1967-71. doi: 10.1073/pnas.76.4.1967. Proc Natl Acad Sci U S A. 1979. PMID: 109836 Free PMC article. - Cistrons encoding Escherichia coli heat-labile toxin.
Dallas WS, Gill DM, Falkow S. Dallas WS, et al. J Bacteriol. 1979 Sep;139(3):850-8. doi: 10.1128/jb.139.3.850-858.1979. J Bacteriol. 1979. PMID: 383697 Free PMC article. - Effects of insertions and deletions in glnG (ntrC) of Escherichia coli on nitrogen regulator I-dependent DNA binding and transcriptional activation.
Shiau SP, Chen P, Reitzer LJ. Shiau SP, et al. J Bacteriol. 1993 Jan;175(1):190-9. doi: 10.1128/jb.175.1.190-199.1993. J Bacteriol. 1993. PMID: 8416895 Free PMC article. - Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903.
Grindley ND, Joyce CM. Grindley ND, et al. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7176-80. doi: 10.1073/pnas.77.12.7176. Proc Natl Acad Sci U S A. 1980. PMID: 6261245 Free PMC article. - Isolation of maxicircle component of kinetoplast DNA from hemoflagellate protozoa.
Simpson L. Simpson L. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1585-8. doi: 10.1073/pnas.76.4.1585. Proc Natl Acad Sci U S A. 1979. PMID: 286999 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1975 Nov;72(11):4280-4 - PubMed
- Nucleic Acids Res. 1974 Jun;1(6):727-52 - PubMed
- J Mol Biol. 1975 Jan 25;91(3):315-28 - PubMed
- Biochemistry. 1975 Aug 26;14(17):3787-94 - PubMed
- Annu Rev Biochem. 1975;44:273-93 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources