Different carbon sources affect lifespan and protein redox state during Saccharomyces cerevisiae chronological ageing - PubMed (original) (raw)
Different carbon sources affect lifespan and protein redox state during Saccharomyces cerevisiae chronological ageing
F Magherini et al. Cell Mol Life Sci. 2009 Mar.
Abstract
In this study, a proteomic approach that combines selective labelling of proteins containing reduced cysteine residues with two-dimensional electrophoresis/mass spectrometry was used to evaluate the redox state of protein cysteines during chronological ageing in Saccharomyces cerevisiae. The procedure was developed on the grounds that biotin-conjugated iodoacetamide (BIAM) specifically reacts with reduced cysteine residues. BIAM-labelled proteins can then be selectively isolated by streptavidin affinity capture. We compared cells grown on 2% glucose in the exponential phase and during chronological ageing and we found that many proteins undergo cysteine oxidation. The target proteins include enzymes involved in glucose metabolism. Both caloric restriction and growth on glycerol resulted in a decrease in the oxidative modification. Furthermore, in these conditions a reduced production of ROS and a more negative glutathione half cell redox potential were observed.
Similar articles
- Extension of chronological lifespan by ScEcl1 depends on mitochondria in Saccharomyces cerevisiae.
Azuma K, Ohtsuka H, Murakami H, Aiba H. Azuma K, et al. Biosci Biotechnol Biochem. 2012;76(10):1938-42. doi: 10.1271/bbb.120427. Epub 2012 Oct 7. Biosci Biotechnol Biochem. 2012. PMID: 23047113 - Involvement of oxidative stress response genes in redox homeostasis, the level of reactive oxygen species, and ageing in Saccharomyces cerevisiae.
Drakulic T, Temple MD, Guido R, Jarolim S, Breitenbach M, Attfield PV, Dawes IW. Drakulic T, et al. FEMS Yeast Res. 2005 Dec;5(12):1215-28. doi: 10.1016/j.femsyr.2005.06.001. Epub 2005 Jul 28. FEMS Yeast Res. 2005. PMID: 16087409 - Glutathione levels influence chronological life span of Saccharomyces cerevisiae in a glucose-dependent manner.
Tello-Padilla MF, Perez-Gonzalez AY, Canizal-García M, González-Hernández JC, Cortes-Rojo C, Olivares-Marin IK, Madrigal-Perez LA. Tello-Padilla MF, et al. Yeast. 2018 May;35(5):387-396. doi: 10.1002/yea.3302. Epub 2018 Feb 1. Yeast. 2018. PMID: 29277922 - Cellular redox homeostasis, reactive oxygen species and replicative ageing in Saccharomyces cerevisiae.
Ayer A, Gourlay CW, Dawes IW. Ayer A, et al. FEMS Yeast Res. 2014 Feb;14(1):60-72. doi: 10.1111/1567-1364.12114. Epub 2013 Nov 8. FEMS Yeast Res. 2014. PMID: 24164795 Review. - Caloric restriction and redox state: does this diet increase or decrease oxidant production?
Kowaltowski AJ. Kowaltowski AJ. Redox Rep. 2011;16(6):237-41. doi: 10.1179/1351000211Y.0000000014. Redox Rep. 2011. PMID: 22195991 Free PMC article. Review.
Cited by
- Proteomic and carbonylation profile analysis of rat skeletal muscles following acute swimming exercise.
Magherini F, Gamberi T, Pietrovito L, Fiaschi T, Bini L, Esposito F, Marini M, Abruzzo PM, Gulisano M, Modesti A. Magherini F, et al. PLoS One. 2013 Aug 13;8(8):e71839. doi: 10.1371/journal.pone.0071839. eCollection 2013. PLoS One. 2013. PMID: 23967250 Free PMC article. - Fructose-Induced Carbonyl/Oxidative Stress in S. cerevisiae: Involvement of TOR.
Valishkevych BV, Vasylkovska RA, Lozinska LM, Semchyshyn HM. Valishkevych BV, et al. Biochem Res Int. 2016;2016:8917270. doi: 10.1155/2016/8917270. Epub 2016 Feb 25. Biochem Res Int. 2016. PMID: 27019749 Free PMC article. - Carbon Sources for Yeast Growth as a Precondition of Hydrogen Peroxide Induced Hormetic Phenotype.
Vasylkovska R, Petriv N, Semchyshyn H. Vasylkovska R, et al. Int J Microbiol. 2015;2015:697813. doi: 10.1155/2015/697813. Epub 2015 Dec 30. Int J Microbiol. 2015. PMID: 26843865 Free PMC article. - Time line of redox events in aging postmitotic cells.
Brandes N, Tienson H, Lindemann A, Vitvitsky V, Reichmann D, Banerjee R, Jakob U. Brandes N, et al. Elife. 2013 Feb 5;2:e00306. doi: 10.7554/eLife.00306. Elife. 2013. PMID: 23390587 Free PMC article. - Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster.
Menger KE, James AM, Cochemé HM, Harbour ME, Chouchani ET, Ding S, Fearnley IM, Partridge L, Murphy MP. Menger KE, et al. Cell Rep. 2015 Jun 30;11(12):1856-65. doi: 10.1016/j.celrep.2015.05.033. Epub 2015 Jun 18. Cell Rep. 2015. PMID: 26095360 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases