Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC - PubMed (original) (raw)

Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC

B Bartholomew et al. Mol Cell Biol. 1991 Oct.

Abstract

A novel photocrosslinking method has been used to identify the components of transcription factor IIIB (TFIIIB) and TFIIIC that associate with DNA upstream of the Saccharomyces cerevisiae SUP4 tRNATyr gene and to map these components to specific positions in DNA. When TFIIIC binds to the tRNA gene, only its second-largest subunit (135 kDa) is accessible for reaction with a photoactive nucleotide, 5-[N-(p-azidobenzoyl)-3-aminoallyl]-dUMP, inserted into DNA upstream of the transcriptional start. Formation of TFIII(C + B)-tRNA gene complexes specifically brings two additional polypeptides (90 and 70 kDa) within reach of upstream photoprobes. A collection of 13 probes has been used to map the locations of these three proteins along a 45-bp segment of DNA upstream of the transcriptional start site. Evidence is presented that the 90- and 70-kDa polypeptides are separate and distinct components of yeast TFIIIB, that they are accessible to crosslinking on opposite sides of the DNA helix in a 6-bp segment centered 35 bp upstream of the tRNATyr gene transcriptional start, and that they interact with the second-largest subunit of TFIIIC.

PubMed Disclaimer

Cited by

TFIIIB placement on a yeast U6 RNA gene in vivo is directed primarily by TFIIIC rather than by sequence-specific DNA contacts.
Gerlach VL, Whitehall SK, Geiduschek EP, Brow DA. Gerlach VL, et al. Mol Cell Biol. 1995 Mar;15(3):1455-66. doi: 10.1128/MCB.15.3.1455. Mol Cell Biol. 1995. PMID: 7862139 Free PMC article.
The TFIIIB-assembling subunit of yeast transcription factor TFIIIC has both tetratricopeptide repeats and basic helix-loop-helix motifs.
Marck C, Lefebvre O, Carles C, Riva M, Chaussivert N, Ruet A, Sentenac A. Marck C, et al. Proc Natl Acad Sci U S A. 1993 May 1;90(9):4027-31. doi: 10.1073/pnas.90.9.4027. Proc Natl Acad Sci U S A. 1993. PMID: 8387209 Free PMC article.
Regulated expression of a mammalian nonsense suppressor tRNA gene in vivo and in vitro using the lac operator/repressor system.
Syroid DE, Tapping RI, Capone JP. Syroid DE, et al. Mol Cell Biol. 1992 Oct;12(10):4271-8. doi: 10.1128/mcb.12.10.4271-4278.1992. Mol Cell Biol. 1992. PMID: 1406620 Free PMC article.
A novel upstream RNA polymerase III promoter element becomes essential when the chromatin structure of the yeast U6 RNA gene is altered.
Martin MP, Gerlach VL, Brow DA. Martin MP, et al. Mol Cell Biol. 2001 Oct;21(19):6429-39. doi: 10.1128/MCB.21.19.6429-6439.2001. Mol Cell Biol. 2001. PMID: 11533232 Free PMC article.
Two novel dATP analogs for DNA photoaffinity labeling.
Zofall M, Bartholomew B. Zofall M, et al. Nucleic Acids Res. 2000 Nov 1;28(21):4382-90. doi: 10.1093/nar/28.21.4382. Nucleic Acids Res. 2000. PMID: 11058139 Free PMC article.

References

1. Mol Cell Biol. 1989 Jun;9(6):2551-66 - PubMed
1. J Biol Chem. 1989 May 5;264(13):7505-11 - PubMed
1. J Biol Chem. 1990 Mar 25;265(9):5095-103 - PubMed
1. EMBO J. 1990 Jul;9(7):2197-205 - PubMed
1. J Biol Chem. 1991 Mar 15;266(8):5162-8 - PubMed

Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC - PubMed (original) (raw)