nirA, the pathway-specific regulatory gene of nitrate assimilation in Aspergillus nidulans, encodes a putative GAL4-type zinc finger protein and contains four introns in highly conserved regions - PubMed (original) (raw)
Comparative Study
nirA, the pathway-specific regulatory gene of nitrate assimilation in Aspergillus nidulans, encodes a putative GAL4-type zinc finger protein and contains four introns in highly conserved regions
G Burger et al. Mol Cell Biol. 1991 Nov.
Abstract
The nucleotide sequence of nirA, mediating nitrate induction in Aspergillus nidulans, has been determined. Alignment of the cDNA and the genomic DNA sequence indicates that the gene contains four introns and encodes a protein of 892 amino acids. The deduced NIRA protein displays all characteristics of a transcriptional activator. A putative double-stranded DNA-binding domain in the amino-terminal part comprises six cysteine residues, characteristic for the GAL4 family of zinc finger proteins. An amino-terminal highly acidic region and two proline-rich regions are also present. The nucleotide sequences of two mutations were determined after they were mapped by transformation with overlapping DNA fragments, amplified by the polymerase chain reaction. nirA87, a mutation conferring noninducibility by nitrate and nitrite, has a -1 frameshift at triplet 340, which eliminates 549 C-terminal amino acids from the polypeptide. Under the assumption that the truncated polypeptide is stable, it comprises the zinc finger domain and the acidic region, which seem not sufficient for transcriptional activation. nirAd-106, an allele conferring nitrogen metabolite derepression of nitrate and nitrite reductase activity, includes two transitions, changing a glutamic acid to a lysine and a valine to an alanine, situated between a basic and a proline-rich region of the protein. Northern (RNA) analysis of the wild type and of constitutive (nirAc) and derepressed (nirAd) mutants show that the nirA transcript does not vary between these strains, being in all cases constitutively expressed. On the other hand, transcript levels of structural genes (niaD and niiA) do vary, being highly inducible in the wild type but constitutively expressed in the nirAc mutant. The nirAd mutant appears phenotypically derepressed, because the niaD and niiA transcript levels are overinduced in the presence of nitrate but are still partially repressed in the presence of ammonium.
Similar articles
- nit-4, a pathway-specific regulatory gene of Neurospora crassa, encodes a protein with a putative binuclear zinc DNA-binding domain.
Yuan GF, Fu YH, Marzluf GA. Yuan GF, et al. Mol Cell Biol. 1991 Nov;11(11):5735-45. doi: 10.1128/mcb.11.11.5735-5745.1991. Mol Cell Biol. 1991. PMID: 1840634 Free PMC article. - Molecular cloning and functional characterization of the pathway-specific regulatory gene nirA, which controls nitrate assimilation in Aspergillus nidulans.
Burger G, Tilburn J, Scazzocchio C. Burger G, et al. Mol Cell Biol. 1991 Feb;11(2):795-802. doi: 10.1128/mcb.11.2.795-802.1991. Mol Cell Biol. 1991. PMID: 1990284 Free PMC article. - Mutational analysis of AREA, a transcriptional activator mediating nitrogen metabolite repression in Aspergillus nidulans and a member of the "streetwise" GATA family of transcription factors.
Wilson RA, Arst HN Jr. Wilson RA, et al. Microbiol Mol Biol Rev. 1998 Sep;62(3):586-96. doi: 10.1128/MMBR.62.3.586-596.1998. Microbiol Mol Biol Rev. 1998. PMID: 9729601 Free PMC article. Review. - On the Evolution of Specificity in Members of the Yeast Amino Acid Transporter Family as Parts of Specific Metabolic Pathways.
Gournas C, Athanasopoulos A, Sophianopoulou V. Gournas C, et al. Int J Mol Sci. 2018 May 8;19(5):1398. doi: 10.3390/ijms19051398. Int J Mol Sci. 2018. PMID: 29738448 Free PMC article. Review.
Cited by
- Regulation of nutrient utilization in filamentous fungi.
Kerkaert JD, Huberman LB. Kerkaert JD, et al. Appl Microbiol Biotechnol. 2023 Oct;107(19):5873-5898. doi: 10.1007/s00253-023-12680-4. Epub 2023 Aug 4. Appl Microbiol Biotechnol. 2023. PMID: 37540250 Free PMC article. Review. - srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans.
Ogawa M, Fukuda R, Iwama R, Koyama Y, Horiuchi H. Ogawa M, et al. Sci Rep. 2023 Mar 15;13(1):4285. doi: 10.1038/s41598-023-31363-8. Sci Rep. 2023. PMID: 36922566 Free PMC article. - Recognition of galactose by a scaffold protein recruits a transcriptional activator for the GAL regulon induction in Candida albicans.
Sun X, Yu J, Zhu C, Mo X, Sun Q, Yang D, Su C, Lu Y. Sun X, et al. Elife. 2023 Feb 1;12:e84155. doi: 10.7554/eLife.84155. Elife. 2023. PMID: 36723430 Free PMC article. - Genomic and Transcriptomic Study for Screening Genes Involved in the Limonene Biotransformation of Penicillium digitatum DSM 62840.
Zhang LL, Huang W, Zhang YY, Fan G, He J, Ren JN, Li Z, Li X, Pan SY. Zhang LL, et al. Front Microbiol. 2020 Apr 22;11:744. doi: 10.3389/fmicb.2020.00744. eCollection 2020. Front Microbiol. 2020. PMID: 32390984 Free PMC article. - Synthetic control devices for gene regulation in Penicillium chrysogenum.
Mózsik L, Büttel Z, Bovenberg RAL, Driessen AJM, Nygård Y. Mózsik L, et al. Microb Cell Fact. 2019 Nov 18;18(1):203. doi: 10.1186/s12934-019-1253-3. Microb Cell Fact. 2019. PMID: 31739777 Free PMC article.
References
- Nucleic Acids Res. 1986 Oct 10;14(19):7767-81 - PubMed
- Nucleic Acids Res. 1986 Jan 10;14(1):455-65 - PubMed
- Proc Natl Acad Sci U S A. 1987 Jul;84(14):4841-5 - PubMed
- Nucleic Acids Res. 1987 Oct 12;15(19):7991-8001 - PubMed
- Methods Enzymol. 1987;153:3-11 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases