Coordinate estrogen-regulated instability of serum protein-coding messenger RNAs in Xenopus laevis - PubMed (original) (raw)
Coordinate estrogen-regulated instability of serum protein-coding messenger RNAs in Xenopus laevis
R L Pastori et al. Mol Endocrinol. 1991 Apr.
Abstract
Estrogen causes the cytoplasmic destabilization of albumin and gamma-fibrinogen mRNA in Xenopus laevis liver. The purpose of the present study was to determine whether mRNA destabilization is a generalized phenomenon in response to estrogen, or whether this process is restricted to a particular class of mRNAs. To address this, we have expanded our bank of serum protein-coding cDNA clones to include transferrin, the second protein of inter-alpha-trypsin inhibitor and clone 12B, for which there is no mammalian homolog. Together with albumin and gamma-fibrinogen, these represent more than 85% of the mRNAs encoding liver secreted proteins. Estrogen administration to male Xenopus or to liver explant cultures causes the generalized disappearance of all of these mRNAs. In contrast, estrogen has no effect on actin, ferritin, or poly(A)-binding protein mRNA, all of which encode intracellular proteins. We have previously demonstrated that albumin mRNA is degraded in both messenger ribonucleoprotein and polysome fractions. Sucrose gradient analysis demonstrates the same pattern for degradation of all other serum protein-coding mRNAs. Estrogen has no effect on the amounts or gradient distribution of actin, ferritin, or poly(A)-binding protein mRNA. We conclude that regulated destabilization of mRNAs encoding secreted proteins is a generalized phenomenon in response to estrogen stimulation of Xenopus liver.
Similar articles
- Extranuclear estrogen-regulated destabilization of Xenopus laevis serum albumin mRNA.
Schoenberg DR, Moskaitis JE, Smith LH Jr, Pastori RL. Schoenberg DR, et al. Mol Endocrinol. 1989 May;3(5):805-14. doi: 10.1210/mend-3-5-805. Mol Endocrinol. 1989. PMID: 2547154 - The estrogen-regulated destabilization of Xenopus albumin mRNA is independent of translation.
Moskaitis JE, Buzek SW, Pastori RL, Schoenberg DR. Moskaitis JE, et al. Biochem Biophys Res Commun. 1991 Jan 31;174(2):825-30. doi: 10.1016/0006-291x(91)91492-u. Biochem Biophys Res Commun. 1991. PMID: 1993075 - Coordinate estrogen induction of vitellogenin and a small serum protein mRNA in Xenopus laevis liver.
Hayward MA, Barton MC, Shapiro DJ. Hayward MA, et al. Mol Cell Endocrinol. 1985 Feb;39(2):91-8. doi: 10.1016/0303-7207(85)90124-8. Mol Cell Endocrinol. 1985. PMID: 3838520 - Xenopus liver ferritin H subunit: cDNA sequence and mRNA production in the liver following estrogen treatment.
Holland LJ, Wall AA, Bhattacharya A. Holland LJ, et al. Biochemistry. 1991 Feb 19;30(7):1965-72. doi: 10.1021/bi00221a033. Biochemistry. 1991. PMID: 1993207 - A new look at ferritin metabolism.
Halliday JW, Ramm GA, Moss D, Powell LW. Halliday JW, et al. Adv Exp Med Biol. 1994;356:149-56. doi: 10.1007/978-1-4615-2554-7_17. Adv Exp Med Biol. 1994. PMID: 7887219 Review. No abstract available.
Cited by
- Mechanisms of endonuclease-mediated mRNA decay.
Schoenberg DR. Schoenberg DR. Wiley Interdiscip Rev RNA. 2011 Jul-Aug;2(4):582-600. doi: 10.1002/wrna.78. Epub 2011 Feb 10. Wiley Interdiscip Rev RNA. 2011. PMID: 21957046 Free PMC article. Review. - Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.
Tomecki R, Dziembowski A. Tomecki R, et al. RNA. 2010 Sep;16(9):1692-724. doi: 10.1261/rna.2237610. Epub 2010 Jul 30. RNA. 2010. PMID: 20675404 Free PMC article. Review. - Approaches for studying PMR1 endonuclease-mediated mRNA decay.
Otsuka Y, Schoenberg DR. Otsuka Y, et al. Methods Enzymol. 2008;448:241-63. doi: 10.1016/S0076-6879(08)02613-X. Methods Enzymol. 2008. PMID: 19111180 Free PMC article. - The 90-kDa heat shock protein stabilizes the polysomal ribonuclease 1 mRNA endonuclease to degradation by the 26S proteasome.
Peng Y, Liu X, Schoenberg DR. Peng Y, et al. Mol Biol Cell. 2008 Feb;19(2):546-52. doi: 10.1091/mbc.e07-08-0774. Epub 2007 Nov 28. Mol Biol Cell. 2008. PMID: 18045990 Free PMC article. - c-Src activates endonuclease-mediated mRNA decay.
Peng Y, Schoenberg DR. Peng Y, et al. Mol Cell. 2007 Mar 9;25(5):779-87. doi: 10.1016/j.molcel.2007.01.026. Mol Cell. 2007. PMID: 17349962 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources