Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking - PubMed (original) (raw)
. 2009 Mar 10;19(5):391-7.
doi: 10.1016/j.cub.2009.01.057. Epub 2009 Feb 19.
Affiliations
- PMID: 19230664
- DOI: 10.1016/j.cub.2009.01.057
Free article
Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking
Hirokazu Tanaka et al. Curr Biol. 2009.
Free article
Erratum in
- Curr Biol. 2014 Dec 1;24(23):2868
Abstract
Endocytic vesicle trafficking is crucial for regulating activity and localization of plasma membrane components, but the process is still poorly genetically defined in plants. Membrane proteins of the PIN-FORMED (PIN) family exhibit polar localization in plant cells and facilitate cellular efflux of the plant hormone auxin, thereby regulating multiple developmental processes. PIN proteins undergo constitutive endocytosis and GNOM ARF GEF-dependent recycling, and their localization is under extensive regulation by developmental and environmental cues. We designed a fluorescence imaging-based screen to identify Arabidopsis thaliana mutants defective in internalization of proteins including PINs from the plasma membrane. We identified three mutant loci, BFA-visualized endocytic trafficking defective1 (ben1) through ben3 that do not efficiently accumulate PIN1-GFP in intracellular compartments after inhibition of recycling and secretion by fungal toxin brefeldin A (BFA). Fine mapping revealed that BEN1 encodes an ARF GEF vesicle trafficking regulator from the functionally uncharacterized BIG class. ben1 mutant has been previously implicated in pathogen response and shows cell polarity, BFA sensitivity, and growth defects. BEN1 is involved in endocytosis of plasma membrane proteins and localizes to early endocytic compartments distinct from GNOM-positive endosomes. Our results identify BEN1 as the ARF GEF mediating early endosomal traffic.
Similar articles
- BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana.
Kitakura S, Adamowski M, Matsuura Y, Santuari L, Kouno H, Arima K, Hardtke CS, Friml J, Kakimoto T, Tanaka H. Kitakura S, et al. Plant Cell Physiol. 2017 Oct 1;58(10):1801-1811. doi: 10.1093/pcp/pcx118. Plant Cell Physiol. 2017. PMID: 29016942 - BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in Arabidopsis.
Tanaka H, Nodzyłski T, Kitakura S, Feraru MI, Sasabe M, Ishikawa T, Kleine-Vehn J, Kakimoto T, Friml J. Tanaka H, et al. Plant Cell Physiol. 2014 Apr;55(4):737-49. doi: 10.1093/pcp/pct196. Epub 2013 Dec 24. Plant Cell Physiol. 2014. PMID: 24369434 Free PMC article. - An ARF-GEF acting at the Golgi and in selective endocytosis in polarized plant cells.
Teh OK, Moore I. Teh OK, et al. Nature. 2007 Jul 26;448(7152):493-6. doi: 10.1038/nature06023. Nature. 2007. PMID: 17653191 - Role of the GNOM gene in Arabidopsis apical-basal patterning--From mutant phenotype to cellular mechanism of protein action.
Richter S, Anders N, Wolters H, Beckmann H, Thomann A, Heinrich R, Schrader J, Singh MK, Geldner N, Mayer U, Jürgens G. Richter S, et al. Eur J Cell Biol. 2010 Feb-Mar;89(2-3):138-44. doi: 10.1016/j.ejcb.2009.11.020. Epub 2009 Dec 29. Eur J Cell Biol. 2010. PMID: 20036441 Review. - Plant endosomal trafficking pathways.
Reyes FC, Buono R, Otegui MS. Reyes FC, et al. Curr Opin Plant Biol. 2011 Dec;14(6):666-73. doi: 10.1016/j.pbi.2011.07.009. Epub 2011 Aug 5. Curr Opin Plant Biol. 2011. PMID: 21821464 Review.
Cited by
- Developmental patterning function of GNOM ARF-GEF mediated from the cell periphery.
Adamowski M, Matijević I, Friml J. Adamowski M, et al. Elife. 2024 Feb 21;13:e68993. doi: 10.7554/eLife.68993. Elife. 2024. PMID: 38381485 Free PMC article. - Plant-Pathogen Effectors: Cellular Probes Interfering with Plant Defenses in Spatial and Temporal Manners.
Toruño TY, Stergiopoulos I, Coaker G. Toruño TY, et al. Annu Rev Phytopathol. 2016 Aug 4;54:419-41. doi: 10.1146/annurev-phyto-080615-100204. Epub 2016 Jan 17. Annu Rev Phytopathol. 2016. PMID: 27359369 Free PMC article. Review. - A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis.
Singh MK, Richter S, Beckmann H, Kientz M, Stierhof YD, Anders N, Fäßler F, Nielsen M, Knöll C, Thomann A, Franz-Wachtel M, Macek B, Skriver K, Pimpl P, Jürgens G. Singh MK, et al. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795. eCollection 2018 Nov. PLoS Genet. 2018. PMID: 30439956 Free PMC article. - Involvement of BIG5 and BIG3 in BRI1 Trafficking Reveals Diverse Functions of BIG-subfamily ARF-GEFs in Plant Growth and Gravitropism.
Xue S, Zou J, Liu Y, Wang M, Zhang C, Le J. Xue S, et al. Int J Mol Sci. 2019 May 11;20(9):2339. doi: 10.3390/ijms20092339. Int J Mol Sci. 2019. PMID: 31083521 Free PMC article. - PIN auxin efflux carrier polarity is regulated by PINOID kinase-mediated recruitment into GNOM-independent trafficking in Arabidopsis.
Kleine-Vehn J, Huang F, Naramoto S, Zhang J, Michniewicz M, Offringa R, Friml J. Kleine-Vehn J, et al. Plant Cell. 2009 Dec;21(12):3839-49. doi: 10.1105/tpc.109.071639. Epub 2009 Dec 29. Plant Cell. 2009. PMID: 20040538 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous