The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments - PubMed (original) (raw)
Guideline
doi: 10.1373/clinchem.2008.112797. Epub 2009 Feb 26.
Vladimir Benes, Jeremy A Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W Pfaffl, Gregory L Shipley, Jo Vandesompele, Carl T Wittwer
Affiliations
- PMID: 19246619
- DOI: 10.1373/clinchem.2008.112797
Guideline
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments
Stephen A Bustin et al. Clin Chem. 2009 Apr.
Abstract
Background: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments.
Content: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement.
Summary: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results.
Comment in
- Primer sequence disclosure: a clarification of the MIQE guidelines.
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. Bustin SA, et al. Clin Chem. 2011 Jun;57(6):919-21. doi: 10.1373/clinchem.2011.162958. Epub 2011 Mar 18. Clin Chem. 2011. PMID: 21421813 No abstract available. - MIQE: A Step Toward More Robust and Reproducible Quantitative PCR.
Bustin SA, Wittwer CT. Bustin SA, et al. Clin Chem. 2017 Sep;63(9):1537-1538. doi: 10.1373/clinchem.2016.268953. Epub 2017 Jun 12. Clin Chem. 2017. PMID: 28606913 Review. No abstract available.
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