A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA - PubMed (original) (raw)
A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA
P Descombes et al. Cell. 1991.
Abstract
LAP, a transcriptional activator, and LIP, a transcriptional repressor, are translated from a single mRNA species by using two AUGs within the same reading frame. These two proteins share the 145 C-terminal amino acids that contain the basic DNA-binding domain and the leucine zipper dimerization helix. Probably owing to its higher affinity for its DNA cognate sequences, LIP can attenuate the transcriptional stimulation by LAP in substoichiometric amounts. As revealed by transient transfection experiments, a moderate increase in the LAP/LIP ratio results in a significantly higher transcriptional activation of an appropriate target gene. The LAP/LIP ratio increases about 5-fold during terminal rat liver differentiation and is thus likely to modulate the activity of LAP in the intact animal.
Similar articles
- CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials.
Ossipow V, Descombes P, Schibler U. Ossipow V, et al. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8219-23. doi: 10.1073/pnas.90.17.8219. Proc Natl Acad Sci U S A. 1993. PMID: 8367486 Free PMC article. - P53 regulates CCAAT/Enhancer binding protein β gene expression.
Hu B, Liu T, Wu Z, Phan SH. Hu B, et al. Gene. 2023 Oct 30;884:147675. doi: 10.1016/j.gene.2023.147675. Epub 2023 Aug 2. Gene. 2023. PMID: 37541559 - Regulation of liver-specific gene expression.
Noda C, Ichihara A. Noda C, et al. Cell Struct Funct. 1993 Aug;18(4):189-94. doi: 10.1247/csf.18.189. Cell Struct Funct. 1993. PMID: 8293495 Review. No abstract available.
Cited by
- Ribosome rescue factor PELOTA modulates translation start site choice for C/EBPα protein isoforms.
Fernandez SG, Ferguson L, Ingolia NT. Fernandez SG, et al. Life Sci Alliance. 2024 May 21;7(7):e202302501. doi: 10.26508/lsa.202302501. Print 2024 Jul. Life Sci Alliance. 2024. PMID: 38803235 Free PMC article. - Bile acid metabolism and signaling in health and disease: molecular mechanisms and therapeutic targets.
Fleishman JS, Kumar S. Fleishman JS, et al. Signal Transduct Target Ther. 2024 Apr 26;9(1):97. doi: 10.1038/s41392-024-01811-6. Signal Transduct Target Ther. 2024. PMID: 38664391 Free PMC article. Review. - Transcription factor C/EBPα is required for the development of Ly6Chi monocytes but not Ly6Clo monocytes.
Kim S, Chen J, Ou F, Liu TT, Jo S, Gillanders WE, Murphy TL, Murphy KM. Kim S, et al. Proc Natl Acad Sci U S A. 2024 Apr 9;121(15):e2315659121. doi: 10.1073/pnas.2315659121. Epub 2024 Apr 2. Proc Natl Acad Sci U S A. 2024. PMID: 38564635 Free PMC article. - Homodimer-mediated phosphorylation of C/EBPα-p42 S16 modulates acute myeloid leukaemia differentiation through liquid-liquid phase separation.
Wang D, Sun T, Xia Y, Zhao Z, Sheng X, Li S, Ma Y, Li M, Su X, Zhang F, Li P, Ma D, Ye J, Lu F, Ji C. Wang D, et al. Nat Commun. 2023 Oct 30;14(1):6907. doi: 10.1038/s41467-023-42650-3. Nat Commun. 2023. PMID: 37903757 Free PMC article. - Systematic identification of gene combinations to target in innate immune cells to enhance T cell activation.
Xia L, Komissarova A, Jacover A, Shovman Y, Arcila-Barrera S, Tornovsky-Babeay S, Jaya Prakashan MM, Nasereddin A, Plaschkes I, Nevo Y, Shiff I, Yosefov-Levi O, Izhiman T, Medvedev E, Eilon E, Wilensky A, Yona S, Parnas O. Xia L, et al. Nat Commun. 2023 Oct 9;14(1):6295. doi: 10.1038/s41467-023-41792-8. Nat Commun. 2023. PMID: 37813864 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials