N-methyl-D-aspartate 2b receptor subtype (NR2B) promoter methylation in patients during alcohol withdrawal - PubMed (original) (raw)

. 2009 May;116(5):615-22.

doi: 10.1007/s00702-009-0212-2. Epub 2009 Apr 7.

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N-methyl-D-aspartate 2b receptor subtype (NR2B) promoter methylation in patients during alcohol withdrawal

Teresa Biermann et al. J Neural Transm (Vienna). 2009 May.

Abstract

NMDA receptors and especially the NR2B receptor subtype play a crucial role during chronic ethanol consumption and alcohol withdrawal. Therefore, the NR2B receptor subtype expression in peripheral blood cells of 32 male patients suffering from alcohol dependency were assessed through quantitative RT-PCR and to explore regulating epigenetic mechanisms, a methylation analysis was conducted using bisulfite sequencing of a fragment of the NR2B promoter region. The expression of the NR2B receptor increased significantly during the first 24 h of withdrawal treatment (day 1; t = 4.1, P = 0.001), and also on and day 3 (t = 2.4; P = 0.029). The severity of alcohol drinking pattern, measured by lifetime drinking and daily ethanol intake, was negatively correlated with the methylation of a defined cluster of five CPG-sites within the NR2B promoter (lifetime drinking: Spearman's rho = -0.55; P = 0.013; daily ethanol intake: rho = -0.46; P = 0.043). These findings might explain the observation of an impact of alcohol consumption patterns on the gravity of withdrawal symptoms.

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References

    1. Alcohol Clin Exp Res. 2001 Oct;25(10):1536-41 - PubMed
    1. Science. 1980 Nov 7;210(4470):604-10 - PubMed
    1. Addiction. 2004 Feb;99(2):251-7 - PubMed
    1. Brain Res Mol Brain Res. 1998 Jul 15;58(1-2):221-4 - PubMed
    1. Neurosci Lett. 2003 Jan 2;335(3):179-82 - PubMed

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