Docosahexaenoic acid regulates serum amyloid A protein to promote lipolysis through down regulation of perilipin - PubMed (original) (raw)

Docosahexaenoic acid regulates serum amyloid A protein to promote lipolysis through down regulation of perilipin

Ya C Wang et al. J Nutr Biochem. 2010 Apr.

Abstract

Docosahexaenoic acid (DHA) increases lipolysis and decreases lipogenesis through several pathways. DHA also enhances the expression of serum amyloid A protein (SAA), a possible lipid metabolism related gene. The question of whether DHA regulates the expression of SAA to affect lipid metabolism and increase lipolysis needs to be demonstrated in human adipocytes. We designed experiments to determine the role of SAA in regulating lipid metabolism in HepG2 cells using microarray technology. In human hepatocytes, recombinant human SAA1 (hSAA1) inhibited the expression of genes related to lipogenesis and promoted the expression of those involved in lipolysis. When human breast adipocytes were treated with hSAA1 or DHA in vitro, the expression of peroxisome proliferator-activated receptor gamma and other lipogenic genes was decreased, whereas the expression of several lipolytic genes was increased. Glycerol release was increased by both SAA and DHA treatments, suggesting that they increased lipolytic activity in human adipocytes. The expression of perilipin, a lipid droplet-protective protein, was decreased, and hormone-sensitive lipase was increased by both of hSAA1 and DHA treatment. We speculate that the mechanism of lipolysis by DHA or SAA is at least partially the result of increased expression of hormone-sensitive lipase and decreased expression of perilipin. Whereas DHA treatment increased expression of hSAA1 in human adipocytes, the DHA-mediated reduction in expression of lipogenesis genes and enhancement of lipolysis may be through the activity of hSAA1. These results may be useful in developing new approaches to reduce body fat deposition.

PubMed Disclaimer

Cited by

Effects of a Fish Oil Rich in Docosahexaenoic Acid on Cardiometabolic Risk Factors and Oxidative Stress in Healthy Rats.
Miralles-Pérez B, Méndez L, Nogués MR, Sánchez-Martos V, Fortuño-Mar À, Ramos-Romero S, Hereu M, Medina I, Romeu M. Miralles-Pérez B, et al. Mar Drugs. 2021 Sep 29;19(10):555. doi: 10.3390/md19100555. Mar Drugs. 2021. PMID: 34677454 Free PMC article.
Modulation of adipocyte differentiation by omega-3 polyunsaturated fatty acids involves the ubiquitin-proteasome system.
Wójcik C, Lohe K, Kuang C, Xiao Y, Jouni Z, Poels E. Wójcik C, et al. J Cell Mol Med. 2014 Apr;18(4):590-9. doi: 10.1111/jcmm.12194. J Cell Mol Med. 2014. PMID: 24834523 Free PMC article.
Microarray evidences the role of pathologic adipose tissue in insulin resistance and their clinical implications.
Mathur SK, Jain P, Mathur P. Mathur SK, et al. J Obes. 2011;2011:587495. doi: 10.1155/2011/587495. Epub 2011 Apr 28. J Obes. 2011. PMID: 21603273 Free PMC article.
Docosahexaenoic acid enhances hepatic serum amyloid A expression via protein kinase A-dependent mechanism.
Tai CC, Chen CY, Lee HS, Wang YC, Li TK, Mersamm HJ, Ding ST, Wang PH. Tai CC, et al. J Biol Chem. 2009 Nov 20;284(47):32239-47. doi: 10.1074/jbc.M109.024661. Epub 2009 Sep 15. J Biol Chem. 2009. PMID: 19755416 Free PMC article.
Adipose triglyceride lipase (ATGL) clone, expression pattern, and regulation by different lipid sources and lipid levels in large yellow croaker (Pseudosciaena crocea R.).
Wang X, Wang Y, Li Y. Wang X, et al. Mar Biotechnol (NY). 2013 Apr;15(2):197-205. doi: 10.1007/s10126-012-9477-9. Epub 2012 Jul 27. Mar Biotechnol (NY). 2013. PMID: 22836232

Docosahexaenoic acid regulates serum amyloid A protein to promote lipolysis through down regulation of perilipin - PubMed (original) (raw)