The BAR domain superfamily: membrane-molding macromolecules - PubMed (original) (raw)

Figure 1. Cellular Structures and BAR Domain Proteins

(A) Plasma membrane tubules in cells demonstrating the shape-based scaffolding properties of the F-BAR structure. Cells expressing GFP-FBP17 visualized by fluorescence (top left), by transmission electron microscopy (TEM; bottom left), and by cryo-EM-derived single particle reconstruction of an F-BAR-coated tubule generated in vitro (bottom and top right) (Shimada et al., 2007, Frost et al., 2008). (B) I-BAR domains of the missing-in-metastasis (MIM) protein localize to the plasma membrane and filopodia (Mattila et al., 2007; originally published in JCB 176, 953–964). (C) TEM of a Drosophila neuromuscular synapse engineered to overexpress syndapin in muscle, revealing an expansion of the subsynaptic reticulum (reprinted with permission from Kumar et al., 2009). (D) (Left) Immunofluorescence of a skeletal muscle frozen section demonstrating muscle amphiphysin-2 (white) along transverse bands flanking the Z-line. (Right) Frozen section showing immunogold-labeled muscle amphiphysin-2 revealing its concentration on a T-tubule (Butler et al., 1997; originally published in JCB 137,1355–1367). (E) TEM of a transformed BHK21 cell cut perpendicular to the substratum to demonstrate the presence of tubular membrane invaginations in a podosome (Ochoa et al., 2000; originally published in JCB 150, 377–389). (F1) Negative staining of liposomes incubated with amphiphysin-1 and a clathrin coat fraction (reprinted with permission from Macmillan Publishers Ltd: Takei et al., Nat. Cell Biol. 1, 33–39, 1999; copyright 1999). (F2) TEM of the synapse of a neuron deficient in dynamin-1, showing clathrin-coated endocytic pits originating from a plasma membrane tubular invagination ~30 nm in diameter (from Ferguson et al., 2007, Science 316, 570–574; reprinted with permission from AAAS). (F3) TEM showing an endocytic coated pit with a narrow-neck connection to the surface (reprinted from Exp. Cell Res., Goldenthal et al., 1984, 152, pp. 558–564, copyright 1984; with permission from Elsevier). (F4) GFP-GRAF1 endocytic tubules turn over in ~10 min (Lundmark et al., 2008). (F5) TEM of immunogold-labeled glycosyl phosphatidylinositol (GPI)-anchored proteins internalized via a putative clathrin- and dynamin-independent invagination (reprinted with permission from Macmillan Publishers Ltd: Mayor and Pagano, Nat. Rev. Mol. Cell Biol. 8, 603–612, 2007; copyright 2007). The invagination is like that induced by GRAF1 (Lundmark et al., 2008).