Fragments of the internal transcribed spacer 1 of pre-rRNA accumulate in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1 - PubMed (original) (raw)

Fragments of the internal transcribed spacer 1 of pre-rRNA accumulate in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1

A Stevens et al. J Bacteriol. 1991 Nov.

Abstract

The portion of the internal transcribed spacer 1 found on 20S pre-rRNA accumulates in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1, showing that an endonucleolytic cleavage at the 3' terminus of 18S rRNA is involved in the 20S pre-rRNA to 18S mature rRNA conversion. Smaller fragments of the spacer sequence are also found. The exoribonuclease may be involved as a cytoplasmic RNase in the hydrolysis of the spacer.

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References

1. 1. Methods Enzymol. 1989;180:96-109 - PubMed
2. 1. Mol Cell Biol. 1989 Oct;9(10):4422-31 - PubMed
3. 1. Gene. 1990 Oct 30;95(1):85-90 - PubMed
4. 1. Genetics. 1990 Dec;126(4):799-812 - PubMed
5. 1. Methods Enzymol. 1991;194:423-8 - PubMed

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