Upstream induction sequence, the cis-acting element required for response to the allantoin pathway inducer and enhancement of operation of the nitrogen-regulated upstream activation sequence in Saccharomyces cerevisiae - PubMed (original) (raw)
Comparative Study
Upstream induction sequence, the cis-acting element required for response to the allantoin pathway inducer and enhancement of operation of the nitrogen-regulated upstream activation sequence in Saccharomyces cerevisiae
H J van Vuuren et al. J Bacteriol. 1991 Nov.
Abstract
Expression of the DAL2, DAL4, DAL7, DUR1,2, and DUR3 genes in Saccharomyces cerevisiae is induced by the presence of allophanate, the last intermediate of the allantoin degradative pathway. Analysis of the DAL7 5'-flanking region identified an element, designated the DAL upstream induction sequence (DAL UIS), required for response to inducer. The operation of this cis-acting element requires functional DAL81 and DAL82 gene products. We determined the DAL UIS structure by using saturation mutagenesis. A specific dodecanucleotide sequence is the minimum required for response of reporter gene transcription to inducer. There are two copies of the sequence in the 5'-flanking region of the DAL7 gene. There are one or more copies of the sequence upstream of each allantoin pathway gene that responds to inducer. The sequence is also found 5' of the allophanate-inducible CAR2 gene as well. No such sequences were detected upstream of allantoin pathway genes that do not respond to the presence of inducer. We also demonstrated that the presence of a UIS element adjacent to the nitrogen-regulated upstream activation sequence significantly enhances its operation.
Similar articles
- The DAL82 protein of Saccharomyces cerevisiae binds to the DAL upstream induction sequence (UIS).
Dorrington RA, Cooper TG. Dorrington RA, et al. Nucleic Acids Res. 1993 Aug 11;21(16):3777-84. doi: 10.1093/nar/21.16.3777. Nucleic Acids Res. 1993. PMID: 8367295 Free PMC article. - DAL82, a second gene required for induction of allantoin system gene transcription in Saccharomyces cerevisiae.
Olive MG, Daugherty JR, Cooper TG. Olive MG, et al. J Bacteriol. 1991 Jan;173(1):255-61. doi: 10.1128/jb.173.1.255-261.1991. J Bacteriol. 1991. PMID: 1898922 Free PMC article. - A gene product needed for induction of allantoin system genes in Saccharomyces cerevisiae but not for their transcriptional activation.
Bricmont PA, Cooper TG. Bricmont PA, et al. Mol Cell Biol. 1989 Sep;9(9):3869-77. doi: 10.1128/mcb.9.9.3869-3877.1989. Mol Cell Biol. 1989. PMID: 2674683 Free PMC article. - The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression.
Yoo HS, Cooper TG. Yoo HS, et al. Mol Cell Biol. 1989 Aug;9(8):3231-43. doi: 10.1128/mcb.9.8.3231-3243.1989. Mol Cell Biol. 1989. PMID: 2552287 Free PMC article. - Allantoin degradation by Saccharomyces cerevisiae--a model system for gene regulation and metabolic integration.
Cooper TG. Cooper TG. Adv Enzymol Relat Areas Mol Biol. 1984;56:91-139. doi: 10.1002/9780470123027.ch2. Adv Enzymol Relat Areas Mol Biol. 1984. PMID: 6364705 Review. No abstract available.
Cited by
- Urea is a drop-in nitrogen source alternative to ammonium sulphate in Yarrowia lipolytica.
Konzock O, Zaghen S, Fu J, Kerkhoven EJ. Konzock O, et al. iScience. 2022 Dec 1;25(12):105703. doi: 10.1016/j.isci.2022.105703. eCollection 2022 Dec 22. iScience. 2022. PMID: 36567708 Free PMC article. - Nitrogen catabolite repression-sensitive transcription as a readout of Tor pathway regulation: the genetic background, reporter gene and GATA factor assayed determine the outcomes.
Georis I, Feller A, Tate JJ, Cooper TG, Dubois E. Georis I, et al. Genetics. 2009 Mar;181(3):861-74. doi: 10.1534/genetics.108.099051. Epub 2008 Dec 22. Genetics. 2009. PMID: 19104072 Free PMC article. - Recent advances in nitrogen regulation: a comparison between Saccharomyces cerevisiae and filamentous fungi.
Wong KH, Hynes MJ, Davis MA. Wong KH, et al. Eukaryot Cell. 2008 Jun;7(6):917-25. doi: 10.1128/EC.00076-08. Epub 2008 Apr 25. Eukaryot Cell. 2008. PMID: 18441120 Free PMC article. Review. No abstract available. - Dal81 enhances Stp1- and Stp2-dependent transcription necessitating negative modulation by inner nuclear membrane protein Asi1 in Saccharomyces cerevisiae.
Boban M, Ljungdahl PO. Boban M, et al. Genetics. 2007 Aug;176(4):2087-97. doi: 10.1534/genetics.107.075077. Epub 2007 Jul 1. Genetics. 2007. PMID: 17603098 Free PMC article. - A fungal family of transcriptional regulators: the zinc cluster proteins.
MacPherson S, Larochelle M, Turcotte B. MacPherson S, et al. Microbiol Mol Biol Rev. 2006 Sep;70(3):583-604. doi: 10.1128/MMBR.00015-06. Microbiol Mol Biol Rev. 2006. PMID: 16959962 Free PMC article. Review.
References
- Mol Cell Biol. 1982 Dec;2(12):1514-23 - PubMed
- J Mol Biol. 1970 Oct 14;53(1):159-62 - PubMed
- Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
- Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 - PubMed
- J Bacteriol. 1982 Sep;151(3):1237-46 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases