Biochemical transformation of mouse cells by fragments of herpes simplex virus DNA - PubMed (original) (raw)
Biochemical transformation of mouse cells by fragments of herpes simplex virus DNA
N J Maitland et al. Cell. 1977 May.
Abstract
Mouse L cells lacking the enzyme thymidine kinase (LMTK-) have been converted to a TK+ phenotype by infection with fragmented HSV2 strain 333 DNA. The DNA fragments used were either unique, produced by cleavage with the restriction endonucleases Eco RI and Hind III, or randomly produced by mechanical shearing. Survival in HAT medium was used initially to establish the TK+ phenotype; clones possessing the ability to grow in selective medium were picked on the basis of differing morphology and growth rates. Cytosol extracts of these clones possessed virus-specified TK activity identical to that present in cells lytically infected with HSV2, as indicated by thermolability and mobility on polyacrylamide gel electrophoresis. The transformed cells also exhibit HSV-specific immunofluorescence. Based on these transformation studies, it is possible to assign a map location to the TK gene on the HSV genome.
Similar articles
- Virus type-specific thymidine kinase in cells biochemically transformed by herpes simplex virus types 1 and 2.
Davis DB, Munyon W, Buchsbaum R, Chawda R. Davis DB, et al. J Virol. 1974 Jan;13(1):140-5. doi: 10.1128/JVI.13.1.140-145.1974. J Virol. 1974. PMID: 4359421 Free PMC article. - Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.
Wigler M, Silverstein S, Lee LS, Pellicer A, Cheng Yc, Axel R. Wigler M, et al. Cell. 1977 May;11(1):223-32. doi: 10.1016/0092-8674(77)90333-6. Cell. 1977. PMID: 194704 No abstract available. - Biochemical transformation of thymidine kinase (TK)-deficient mouse cells by herpes simplex virus type 1 DNA fragments purified from hybrid plasmids.
Kit S, Otsuka H, Qavi H, Trkula D, Dubbs DR, Hazen M. Kit S, et al. Nucleic Acids Res. 1980 Nov 25;8(22):5233-53. doi: 10.1093/nar/8.22.5233. Nucleic Acids Res. 1980. PMID: 6258139 Free PMC article. - Factors governing expression of the herpes simplex virus gene for thymidine kinase in clonal derivatives of transformed mouse L cells.
Buttyan R, Spear PG. Buttyan R, et al. J Virol. 1981 Jan;37(1):459-72. doi: 10.1128/JVI.37.1.459-472.1981. J Virol. 1981. PMID: 6260994 Free PMC article. - Thymidine kinase gene transfer by herpes simplex virus.
Kraiselburd E. Kraiselburd E. Bull Cancer. 1976 Jul-Sep;63(3):393-8. Bull Cancer. 1976. PMID: 187268 Review.
Cited by
- Lipofection mediated transfection fails for sea urchin coelomocytes.
Barela Hudgell MA, Smith LC. Barela Hudgell MA, et al. PLoS One. 2022 May 6;17(5):e0267911. doi: 10.1371/journal.pone.0267911. eCollection 2022. PLoS One. 2022. PMID: 35522665 Free PMC article. - Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA.
Zelensky AN, Schimmel J, Kool H, Kanaar R, Tijsterman M. Zelensky AN, et al. Nat Commun. 2017 Jul 7;8(1):66. doi: 10.1038/s41467-017-00124-3. Nat Commun. 2017. PMID: 28687761 Free PMC article. - Properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene: mechanisms of reversion to a thymidine kinase-negative phenotype.
Bastow KF, Darby G, Wildy P, Minson AC. Bastow KF, et al. J Virol. 1980 Dec;36(3):746-55. doi: 10.1128/JVI.36.3.746-755.1980. J Virol. 1980. PMID: 16789205 Free PMC article. - Phenotypic switching in cells transformed with the herpes simplex virus thymidine kinase gene.
Ostrander M, Vogel S, Silverstein S. Ostrander M, et al. Mol Cell Biol. 1982 Jun;2(6):708-14. doi: 10.1128/mcb.2.6.708-714.1982. Mol Cell Biol. 1982. PMID: 14582166 Free PMC article. - High-frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion.
Sandri-Goldin RM, Goldin AL, Levine M, Glorioso JC. Sandri-Goldin RM, et al. Mol Cell Biol. 1981 Aug;1(8):743-52. doi: 10.1128/mcb.1.8.743-752.1981. Mol Cell Biol. 1981. PMID: 9279387 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources