Inhibition of latent transforming growth factor-beta1 activation by lentivirus-mediated short hairpin RNA targeting the CD36 gene in NR8383 cells - PubMed (original) (raw)

. 2010 Mar;37(3):1649-55.

doi: 10.1007/s11033-009-9579-2. Epub 2009 Jun 10.

Affiliations

• PMID: 19513812
• DOI: 10.1007/s11033-009-9579-2

Inhibition of latent transforming growth factor-beta1 activation by lentivirus-mediated short hairpin RNA targeting the CD36 gene in NR8383 cells

Xin Wang et al. Mol Biol Rep. 2010 Mar.

Abstract

CD36, a cell surface receptor for thrombospondin-1 (TSP-1), is believed to interact with latent transforming growth factor-beta1 (L-TGF-beta1) thereby activating its fibrogenic bioactivity. In this study, a lentiviral vector expressing a short hairpin RNA (shRNA) targeting the rat CD36 gene (Lv-shCD36) is developed and tested. To observe the inhibitory effect of Lv-shCD36 on the activation of L-TGF-beta1, a rat alveolar macrophage cell line (NR8383), infected with either Lv-shCD36 or Lv-shCD36-NC (non-silenced control lentivirus), was treated with 0.1 microg/ml bleomycin, which is known to stimulate alveolar macrophages to release increasing amounts of TGF-beta1. The results show that Lv-shCD36 can suppress expression of CD36 mRNA and protein in bleomycin-treated NR8383 cells. By quantifying active and total TGF-beta1 in the supernatant, it was discovered that the quantity of total TGF-beta1 is not significantly different between the three groups, while the quantity and percent of active TGF-beta1 in the Lv-shCD36 group was significantly lower than in either the bleomycin-treated group or the Lv-shCD36-NC group, respectively (P < 0.05). These results suggest that Lv-shCD36 can inhibit activation of L-TGF-beta1 secreted in bleomycin-treated NR8383 cells by decreasing the expression of CD36 on the cell membrane, thereby reducing binding of CD36 to TSP-1.

PubMed Disclaimer

Similar articles

• Silencing CD36 gene expression results in the inhibition of latent-TGF-beta1 activation and suppression of silica-induced lung fibrosis in the rat.
  Wang X, Chen Y, Lv L, Chen J. Wang X, et al. Respir Res. 2009 May 13;10(1):36. doi: 10.1186/1465-9921-10-36. Respir Res. 2009. PMID: 19439069 Free PMC article.
• [The inhibitory effect of latent transforming growth factor β1 activation and silicosis by CD36 targeted RNA interference in silicosis model of rat].
  Wang X, Wang YR, Yang DY, Zhang M. Wang X, et al. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2013 Jul;31(7):518-21. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2013. PMID: 24053919 Chinese.
• Activation of rat alveolar macrophage-derived latent transforming growth factor beta-1 by plasmin requires interaction with thrombospondin-1 and its cell surface receptor, CD36.
  Yehualaeshet T, O'Connor R, Green-Johnson J, Mai S, Silverstein R, Murphy-Ullrich JE, Khalil N. Yehualaeshet T, et al. Am J Pathol. 1999 Sep;155(3):841-51. doi: 10.1016/s0002-9440(10)65183-8. Am J Pathol. 1999. PMID: 10487979 Free PMC article.
• A TSP-1 functional fragment inhibits activation of latent transforming growth factor-beta1 derived from rat alveolar macrophage after bleomycin treatment.
  Chen Y, Wang X, Weng D, Tao S, Lv L, Chen J. Chen Y, et al. Exp Toxicol Pathol. 2009 Jan;61(1):67-73. doi: 10.1016/j.etp.2008.06.007. Epub 2008 Aug 21. Exp Toxicol Pathol. 2009. PMID: 18722097
• Induction of TGF-β1 synthesis by macrophages in response to apoptotic cells requires activation of the scavenger receptor CD36.
  Xiong W, Frasch SC, Thomas SM, Bratton DL, Henson PM. Xiong W, et al. PLoS One. 2013 Aug 2;8(8):e72772. doi: 10.1371/journal.pone.0072772. Print 2013. PLoS One. 2013. PMID: 23936544 Free PMC article.

Cited by

• Thrombospondin 1 inhibits inflammatory lymphangiogenesis by CD36 ligation on monocytes.
  Cursiefen C, Maruyama K, Bock F, Saban D, Sadrai Z, Lawler J, Dana R, Masli S. Cursiefen C, et al. J Exp Med. 2011 May 9;208(5):1083-92. doi: 10.1084/jem.20092277. Epub 2011 May 2. J Exp Med. 2011. PMID: 21536744 Free PMC article.
• Human CD1c+ dendritic cells drive the differentiation of CD103+ CD8+ mucosal effector T cells via the cytokine TGF-β.
  Yu CI, Becker C, Wang Y, Marches F, Helft J, Leboeuf M, Anguiano E, Pourpe S, Goller K, Pascual V, Banchereau J, Merad M, Palucka K. Yu CI, et al. Immunity. 2013 Apr 18;38(4):818-30. doi: 10.1016/j.immuni.2013.03.004. Epub 2013 Apr 4. Immunity. 2013. PMID: 23562160 Free PMC article.
• A natural process of cirrhosis resolution and deceleration of liver regeneration after thioacetamide withdrawal in a rat model.
  Gu K, Zhao JD, Ren ZG, Ma NY, Lai ST, Wang J, Liu J, Jiang GL. Gu K, et al. Mol Biol Rep. 2011 Mar;38(3):1687-96. doi: 10.1007/s11033-010-0281-1. Epub 2010 Oct 8. Mol Biol Rep. 2011. PMID: 20931291
• Adenovirus-mediated delivery of shRNA against bFGF mRNA suppresses growth of cultured human primary prostatic stromal cells.
  Wang K, Cheng L, Liang Y, Liu D, Li K, Wang P. Wang K, et al. Mol Biol Rep. 2011 Feb;38(2):971-6. doi: 10.1007/s11033-010-0191-2. Epub 2010 Jun 6. Mol Biol Rep. 2011. PMID: 20526813

References

1. 1. Adv Genet. 2005;54:117-42 - PubMed
2. 1. Hum Gene Ther. 2002 Dec 10;13(18):2197-201 - PubMed
3. 1. J Clin Invest. 1993 Oct;92(4):1812-8 - PubMed
4. 1. Biomol Eng. 2006 Mar;23(1):17-34 - PubMed
5. 1. Am J Respir Cell Mol Biol. 2000 Aug;23(2):204-12 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.
  • Springer

• Miscellaneous

  • NCI CPTAC Assay Portal