Age-related changes in intracellular cytokine expression in healthy children - PubMed (original) (raw)
Comparative Study
doi: 10.1684/ecn.2009.0149.
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- PMID: 19541593
- DOI: 10.1684/ecn.2009.0149
Free article
Comparative Study
Age-related changes in intracellular cytokine expression in healthy children
Verena Wiegering et al. Eur Cytokine Netw. 2009 Jun.
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Abstract
Cytokine production by human lymphocytes from healthy children (ages 0-18 years) was assessed using a flow cytometric procedure involving staining of intracellular cytokines by the paraformaldehyde-saponin procedure. To establish valid cytokine values for intracellular cytokine expression in healthy children in the different age groups, we measured 117 samples after 24 h in vitro stimulation with PMA, ionomycin and brefeldin followed by staining with intracellular anti-cytokine and surface antibodies. We found decreasing IL-2 expression, increasing IFN-gamma and TNF-alpha production and stable IL-4, Ki67 and TGFb levels with advancing age. The cytokines were mainly produced by memory T-cells. Apart from age, there was a differential expression in boys and girls: boys (< 6 years) produce significantly more IL-2 (p < 0,04), while girls > 12 years produce more IFNg than boys of the same age (p < 0.05). This systematic analysis of cytokine profiles during childhood allows a better understanding of immune maturation and will contribute significantly to the interpretation of cytokine data from children with pathological conditions.
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